Cannabis & Cannabinoids · Terpenes · The pine-scented molecules in conifers, rosemary and cannabis

Pinene (Alpha-Pinene and Beta-Pinene)

Q: What is pinene?

Pinene is the name for two pine-smelling plant molecules, alpha-pinene and beta-pinene. Both are monoterpenes with the formula C10H16. They are found in pine and rosemary oils, in other plants and in cannabis.

Q: Are alpha-pinene and beta-pinene the same?

No. They have the same formula but different structures and have been studied separately. Most human work is on alpha-pinene. In mice only beta-pinene was antidepressant-like in one test, and each has two mirror forms that can behave differently.

Q: Which plants contain pinene?

Pine and rosemary oils are rich in alpha-pinene: one Scots pine oil was 39.4% and rosemary oils from six places were 13.5% to 37.7%. It is also in hinoki cypress oil, tea tree oil, turpentine and cannabis. Amounts vary by plant and batch.

Q: Does pinene counter the memory effects of THC?

Not in the one human trial. In 19 healthy adults, inhaled alpha-pinene did not reduce THC memory impairment or change other THC effects. Beta-pinene was not tested. Do not drive after THC.

Q: Does pinene help anxiety, sleep or memory?

Evidence in people is lacking. Mice breathing alpha-pinene showed anxiolytic-like behaviour, and (-)-alpha-pinene by mouth lengthened their non-REM sleep. Rosemary scent changed some memory scores in people, but whole oil was used. It is not a treatment for any condition.

Q: Does pinene open the airways or treat bronchitis?

Not shown for pinene alone. A distillate of alpha-pinene, limonene and 1,8-cineole beat placebo in acute bronchitis, but the effect cannot be credited to pinene. In isolated rat windpipe, pinenes made muscle contract more, and high vapour levels irritated volunteers' airways.

Q: Is pinene an antibiotic?

Only in the lab. The (+) forms of alpha- and beta-pinene killed bacteria and fungi in test tubes at high concentrations that also reduced mouse immune-cell survival, and beta-pinene boosted some antibiotics against Staphylococcus aureus. Do not use it on infections.

Q: Can pinene cause a skin allergy?

Yes. In patch tests of turpentine-allergic people, 17 of 22 reacted to alpha-pinene in Portugal and 8 of 24 pottery workers did. Oxidised terpenes are a recognised cause of contact allergy. If a product makes your skin itch or blister, stop using it and ask a clinician.

Q: Is pinene safe?

It is a permitted food flavour in the US, but concentrated pinene is a hazard: it is flammable, irritates the skin and can be dangerous if swallowed. High vapour levels irritated the airways of volunteers. Long-term safety of vaporized pinene is unknown, and added terpenes raised breakdown products in a lab test of vape and dab aerosol.

Q: Do "pine" strains have more pinene?

We have no evidence that a strain name or smell describes its pinene content. Our lab data show the same strain measured very differently between datasets, and 140 German flowers showed no link between terpene profiles and sativa, indica or hybrid labels.

Alpha-pinene: 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene · Beta-pinene: 6,6-dimethyl-2-methylidenebicyclo[3.1.1]heptane (both C10H16) · also called Alpha-pinene (2-pinene), beta-pinene (2(10)-pinene, nopinene); each exists as (+) and (-) mirror forms

Pinene is the name for two pine-smelling plant molecules (monoterpenes): alpha-pinene and beta-pinene. They are found in pine and rosemary oils and in cannabis. The best human test, in 19 adults, found alpha-pinene did not reduce THC memory impairment. Most other research is in animals, cells or whole oils. Turpentine-type material can cause skin allergy, and swallowing pine oils is dangerous.

General information, not medical advice. Last substantive update October 25, 2026 · Not medically reviewed. A named clinician has not signed off on this page. How this page is made

About Pinene (Alpha-Pinene and Beta-Pinene)

Pinene is two pine-scented molecules, alpha-pinene and beta-pinene, found in pine and rosemary oils, cypress, tea tree oil, turpentine and cannabis. Only alpha-pinene has been tested with THC in people: in a 19-person double-blind trial it did not reduce THC memory impairment or change other THC effects, so the popular claim is not supported. Almost all other research is in mice, cells, whole oils or mixtures, and in those, effects on anxiety, sleep, mood, airways and microbes are early and sometimes point in different directions. Turpentine-type material is a known skin allergen (17 of 22 turpentine-allergic people reacted to alpha-pinene), high vapour levels irritate the airways, and swallowing pine oils is dangerous. The FDA lists both pinenes as permitted synthetic food flavourings. No source read shows that a "pine" strain has more pinene, or that more pinene changes how cannabis feels.

What it is

"Pinene" names two different molecules. Alpha-pinene (2,6,6-trimethylbicyclo[3.1.1]hept-2-ene) and beta-pinene (6,6-dimethyl-2-methylidenebicyclo[3.1.1]heptane) both have the formula C10H16, but they are not the same and have been studied separately. Each also has two mirror-image forms, written (+) and (-), and in the lab only the (+) forms killed microbes in one study. PubChem lists alpha-pinene as compound 6654 and beta-pinene as 14896.

Cannabis makes terpenes in the resin glands of the female flower. In the hemp variety Finola, the plant's enzymes make (+)-alpha-pinene among other terpenes. In one analysis of 33 flower samples, alpha-pinene was 5.8% of total terpenes in CBD-dominant samples and 4.9% in THC-dominant ones; beta-pinene was 5.0% and 5.8%. In a separate study of two strains both pinenes were among the high-amount terpenes.

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Where you meet pinene

Pine and rosemary oils are rich in it. Commercial Scots pine oil was 39.4% alpha-pinene in one study. Rosemary oils from six places were dominated by (+)-alpha-pinene (13.5% to 37.7%) and 1,8-cineole (16.1% to 29.3%), and the make-up of rosemary oil varies a lot between chemotypes. Alpha-pinene is also the main volatile in hinoki cypress oil and one of the major constituents of tea tree oil. Turpentine, a distillate of pine resin, is a mixture that contains alpha-pinene, beta-pinene and 3-carene. This website's pages on pine oil, rosemary oil and rosemary give more on those plants.

Because pinene is also an added food flavour and a fragrance ingredient, a pinene figure on a label does not tell you whether it came from the plant. In a US national survey of 2,213 people, pinenes were measurable in the blood of ordinary people, and recent citrus-rich food went with higher alpha-pinene.

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What the lab numbers show

Our data hold pinene measurements for 175 named strains: 169 have an alpha-pinene value, 164 a beta-pinene value and 158 have both. They include 24 of the 28 strains that have a profile page here. The values come from several kinds of source, each in its own unit, so numbers in different units must not be compared, and alpha- and beta-pinene are always reported separately. A Nevada regulator dataset gives one median per strain in percent by weight (the source does not label the unit per column). For alpha-pinene, 63 strains run from 0.0195% (two strains tie: Dead Head OG, 250 samples, and presoviettahoe, 151 samples) to 0.4085% (Starberry, 247 samples), and the middle strain is about 0.05%. For beta-pinene, 65 strains run from 0.024% to 0.2061% (Starberry again), with a middle of about 0.07%. In both lists 51 of the medians rest on datasets of 100 or more samples. A study of one California dispensary (February 2015 to February 2016) gives means in mg/g from 5 to 19 samples per strain, with the spread (SD) printed: alpha-pinene for 18 strains (0.3 to 4.2 mg/g) and beta-pinene for 30 strains (0.3 to 2.0 mg/g), both highest in Blue Dream. A hemp field trial reports means in ppm for 20 varieties (alpha-pinene 2.91 to 243 ppm, beta-pinene 2.05 to 99.5 ppm). A few papers report single batches in micrograms per gram (5 strains), a table headed percent dry weight with much smaller numbers and an uncertain unit (62 strains), and 15 strains have an alpha-pinene value printed in percent with no sample count. We do not compare those with the other sets.

The same strain name varies a lot. Blue Dream's alpha-pinene is 0.3384% in the Nevada table (a median; 330 of 363 samples had a value), 4.2 mg/g in the dispensary study (mean of 19 samples, SD 1.3), and 0.00064 and 0.00068 as printed for two single batches in the percent-dry-weight table; none of these can be compared with another. Gelato's alpha-pinene is 0.0579% in Nevada (118 of 123 samples) and 1.0 mg/g in the dispensary study (5 samples, SD 0.1). These numbers are measurements of flower content from regulator, lab and published datasets, not from our own testing. They say nothing about effects, and our data do not say whether the (+) or (-) mirror form was measured.

Examples within the same unit and source type (Nevada medians, percent by weight; profile strains): the highest alpha-pinene medians were Blue Dream (0.3384%, 363 samples), Zkittlez (0.3129%, 8 samples, a very small group) and Durban Poison (0.097%, 214 samples). Among profile strains with at least 30 samples that had a value, the lowest were Gorilla Glue #4 (0.0326%, 660 samples), Bubba Kush (0.0345%, 45 samples) and Trainwreck (0.0389%, 30 samples). For beta-pinene, Blue Dream (0.15%) and Durban Poison (0.1331%) were again highest among the well-sampled profile strains, and Bubba Kush (0.032%) was lowest. Sample counts differ a lot, so smaller groups are less certain.

The popular claims

"Pinene cancels the memory problems of THC." The only human trial of this idea found it did not. The authors write that the result is inconsistent with some cannabis industry claims. The idea began as a hypothesis in a 2011 review.

"Pinene opens the airways." The evidence is mixed. A three-terpene distillate helped people with acute bronchitis, but pinene alone was not tested. In isolated rat windpipe, alpha- and beta-pinene made muscle contractions stronger, and volunteers breathing a lot of pinene or turpentine vapour reported airway discomfort.

"Pinene is a natural antibiotic." Only in test tubes, at high concentrations that also harmed mouse immune cells, and only for the (+) forms. No study tested infections in people.

"Piney strains have more pinene or a different feel." A 2025 analysis of 140 German medicinal flowers found no relation between terpene profiles and sativa, indica or hybrid labels or estimated effects. See the lab numbers above for how much one strain name varies.

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What the research shows

Human: a double-blind trial of 19 healthy adults found alpha-pinene (15 mg inhaled) alone matched placebo, and with 30 mg THC it did not reduce memory impairment or change other effects. Rosemary scent changed some memory scores in 144 volunteers, and in 20 volunteers the scores tracked 1,8-cineole in the blood. In 12 men, cypress oil vapour went with more natural killer cell activity and lower urinary stress hormones, without a placebo group. Mixtures that included pinene (a CBD plus eight-terpene mix for sleep, a three-terpene distillate for bronchitis) showed benefits that cannot be credited to pinene. Chamber studies show about 60% of inhaled alpha-pinene is absorbed, and the data suggest it may be stored in body fat.

Animals and cells: in mice, inhaled alpha-pinene gave anxiolytic-like behaviour, (-)-alpha-pinene by mouth lengthened non-REM sleep, and beta-pinene (not alpha) was antidepressant-like in a swimming test. Alpha-pinene protected rat stomach and nerve-like cells, and in lab dishes the (+) pinenes killed microbes and boosted some antibiotics. Alpha-pinene with temozolomide reduced human brain-cancer cell growth in a dish. Rodent studies of inhaled alpha-pinene reported bladder and sperm effects, and its liver metabolite alpha-pinene oxide was mutagenic in bacteria.

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Regulatory status

In the US, section 172.515 of the Code of Federal Regulations lists alpha-pinene and beta-pinene among synthetic flavouring substances that may be safely used in food when used in the minimum amount required and in line with good manufacturing practice (read 2026-10-25). That is a food flavour listing. It does not speak to vaping, heating, concentrated oils or cannabis products.

Companies' notified hazard classes (aggregated by PubChem from European notifications) include flammable liquid, skin irritant, skin sensitiser and aspiration hazard; these are notifications, not a regulator's ruling. We did not read EFSA, JECFA, IFRA or EU cosmetics documents on pinene, so we say nothing about them.

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At a glance

Traditional uses history, not proof

Flavouring and fragrance ingredient: the FDA lists both pinenes among permitted synthetic food flavourings
Component of turpentine, pine oil and many essential oils (pine, rosemary, cypress, tea tree)
Pine and clove oils are commonly used by inhalation (one 2022 paper)
Modern aromatherapy scents; no traditional medicinal use of pinene itself was verified from a source we read

Studied for research

THC memory impairment, one 19-person trial of alpha-pinene: no benefit, against
Anxiety-like behaviour, mouse studies, very low
Sleep, a mouse study and a nine-ingredient mixture trial, very low
Memory with rosemary scent, whole-oil trials, very low
Low mood-like behaviour, mouse studies of beta-pinene, very low
Bronchitis: a three-terpene mixture trial; airways in animals, mixed, very low
Killing microbes in the lab, very low
Cancer cells, stomach protection, immune cells: lab or animal work, very low to insufficient

Main compounds

Alpha-pinene (CID 6654), with (+) form CID 82227 and (-) form CID 440968
Beta-pinene (CID 14896)
Metabolites: alpha-pinene oxide, cis- and trans-verbenol
Related turpentine and oil terpenes: 3-carene, 1,8-cineole, limonene

Common forms

Natural part of cannabis flower and extracts
Pine, rosemary, cypress and tea tree essential oils
Turpentine and pine-oil cleaners (not for swallowing or skin)
Pure compounds in lab trials: vaporized alpha-pinene, oral mixtures

Research links: PubChem: alpha-pinene · PubChem: beta-pinene · US eCFR: 21 CFR 172.515 (flavouring substances) · Alpha-pinene and THC memory trial (PubMed) · Research Library · Genes & Biomarkers Library

What it is and its history

Name: Pinene (two different molecules: alpha-pinene and beta-pinene)
Chemical name: Alpha-pinene: 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene. Beta-pinene: 6,6-dimethyl-2-methylidenebicyclo[3.1.1]heptane.
Other names: alpha-Pinene; 2-Pinene; pin-2(3)-ene (alpha); (+)-alpha-pinene and (-)-alpha-pinene (mirror forms); beta-Pinene; 2(10)-Pinene; Pin-2(10)-ene; Nopinene (beta)
Common names: Pinene (alpha-pinene, beta-pinene) (English); Pineno (alfa-pineno, beta-pineno) (Spanish)
Formula: C10H16
Molecular weight: 136.23
CAS number: 80-56-8 (alpha-pinene); 127-91-3 (beta-pinene)
PubChem ID: 6654
Chemical class: Monoterpene: a small aromatic hydrocarbon built from ten carbon atoms with a two-ring (bicyclic) frame and no oxygen. The two pinenes differ in where the double bond sits.
How the plant makes it: Cannabis makes terpenes in the resin glands (trichomes) of the female flower. In the hemp variety Finola, nine terpene-making enzymes were identified, and their products include (+)-alpha-pinene along with beta-myrcene, (E)-beta-ocimene, (-)-limonene, beta-caryophyllene and alpha-humulene. The abstract does not name an enzyme for beta-pinene. A review notes that terpenes and cannabinoids share a precursor. Which mirror form of each pinene other cannabis varieties make was not examined in the abstracts read.
Where it is found: Cannabis flower: in one analysis of 33 samples, alpha-pinene was 5.8% (CBD-dominant) and 4.9% (THC-dominant) of total terpenes, and beta-pinene 5.0% and 5.8%. In another study of two strains both were among the high-amount terpenes.; Rosemary essential oil: from six places of origin, the oils were dominated by (+)-alpha-pinene (13.5% to 37.7%) and 1,8-cineole (16.1% to 29.3%); at least five chemotypes exist.; Scots pine (Pinus sylvestris) essential oil: alpha-pinene was 39.4% in one commercial oil.; Hinoki cypress (Chamaecyparis obtusa) oil: alpha-pinene is its main volatile component, and alpha- and beta-pinene were detected in room air when its vapour was used.; Tea tree oil: alpha-pinene is one of its major constituents, according to a review.; Turpentine (pine oleoresin distillate): a mixture of monoterpenes that includes alpha-pinene, beta-pinene and 3-carene.; Everyday exposure: alpha-pinene, beta-pinene and limonene were measurable in the blood of 2,213 people in a US survey; citrus-rich food intake went with higher alpha-pinene.
Related or similar compounds: Name: Limonene; Note: A different cannabis and citrus monoterpene (C10H16) with a ring and a different smell. See the limonene profile.; Name: 3-Carene (delta-3-carene); Note: PubChem CID 26049, C10H16. Found in some turpentine and a main culprit in Scandinavian turpentine allergy. Not the same as the pinenes.; Name: Pine tar oil, pine oil and turpentine; Note: Products, not molecules. They contain pinenes along with many other components.; Name: Alpha-pinene versus beta-pinene mirror forms; Note: Each pinene exists as (+) and (-) forms that are mirror images. In the lab only the (+) forms killed microbes in one study, while the (-) form of alpha-pinene acted on sleep receptors in mice. The two forms are different molecules for research purposes.

Traditional use and history history, not clinical proof

Tradition or era	Documented use	Source
Turpentine and skin allergy	A 2021 review says turpentine caused occupational contact dermatitis in painters from the early 20th century, and that ending industrial exposure stopped the skin sensitisation in those workers.	Karlberg and Lepoittevin 2021 (PubMed 34453446)
Inhaled pine oil	A 2022 paper says Scots pine and clove essential oils are commonly used by inhalation.	Csikos et al 2022 (PubMed 35744988)
Cannabis entourage hypothesis	A 2011 review of cannabis terpenes, including alpha-pinene, proposed that terpenes may add to the effects of cannabis and presented evidence for plant components as "putative antidotes" to the intoxicating effects of THC. It was a proposal to be tested.	Russo 2011 (PubMed 21749363)
First human test of the memory idea	In 2025 a double-blind crossover trial tested inhaled alpha-pinene against THC memory impairment and found no mitigation.	Kumar et al 2025 (PubMed 40734690)
Food flavouring listing	The FDA lists alpha-pinene and beta-pinene among synthetic flavouring substances that may be safely used in food under stated conditions (21 CFR 172.515, text dated 2026-10-01).	21 CFR 172.515 via eCFR
Traditional medicinal use (not verified)	No source we read documents a traditional medicinal use of pinene itself, so none is claimed here (NOT verified).	None read

History shows how a substance was used or discovered. It does not show that a product works or is safe today.

What is inside, and how it may work

What it contains and how those compounds act in cells and animals. Laboratory and animal mechanisms are not proven effects in people.

Main compounds

Compound	Family	Where it is concentrated	Activity studied	Level of evidence
(+)-alpha-Pinene PubChem 82227	Monoterpene (bicyclic hydrocarbon)	Rosemary oil (13.5% to 37.7% as (+)-alpha-pinene), Scots pine oil (39.4% alpha-pinene), cannabis resin Varies by plant and batch; see "What the lab numbers show" on the Overview tab for cannabis measurements.	Active against microbes in the lab; the form made by the Finola hemp enzymes. The abstract of the human trial of alpha-pinene with THC does not state which mirror form was used. ^{33 1 37 17}	Mixed
(-)-alpha-Pinene PubChem 440968	Monoterpene (bicyclic hydrocarbon)	Not examined in the abstracts read	Lengthened non-REM sleep in mice through the GABA-A benzodiazepine site; inactive against microbes in one lab study. ^{43 33}	Animal research
Beta-pinene PubChem 14896	Monoterpene (bicyclic hydrocarbon)	Cannabis resin; turpentine; room air with cypress oil Varies by plant and batch.	Antidepressant-like in mouse swimming tests (alpha-pinene was not); strengthened beta-lactam antibiotics against S. aureus in the lab. Mirror forms differ in toxicity in a brine shrimp screen. ^{10 9 44 13}	Animal research
Alpha-pinene oxide PubChem 91508	Epoxide breakdown product (metabolite)	Not a plant compound in the sources read; formed in the body and in the lab from alpha-pinene	Formed in rat and human liver preparations; mutagenic in bacteria at 25 micrograms per plate and above. ^{40 41}	Laboratory research
Verbenols (cis- and trans-verbenol)	Oxygenated breakdown products (metabolites)	Not a plant compound in the sources read	About 1% to 4% of inhaled alpha-pinene was passed in urine as cis- and trans-verbenol, mostly within 20 hours. ¹⁹	Human data
3-Carene (delta-3-carene) PubChem 26049	Monoterpene	Some turpentine	Its hydroperoxides were the main culprit in Scandinavian turpentine allergy. ^{14 6}	Human data
1,8-Cineole (eucalyptol) PubChem 2758	Oxygenated monoterpene	Rosemary oil (16.1% to 29.3% in six origins)	The compound whose blood level tracked cognitive scores after rosemary aroma, and protected nerve-like cells with alpha-pinene in a dish. ^{26 31 37}	Mixed
d-Limonene PubChem 440917	Monoterpene	Citrus peel oils; cannabis; with alpha-pinene in the bronchitis distillate	A separate cannabis terpene with its own profile page here; part of the three-terpene bronchitis distillate. ²³	Mixed

Mechanisms, receptors and pathways

Target or pathway	Gene or receptor	What was found	Level
Benzodiazepine site of the GABA-A receptor	GABA-A receptor (alpha-1 and gamma-2 subunits)	In mice, (-)-alpha-pinene by mouth lengthened non-REM sleep. In brain slices it strengthened GABA-A-mediated nerve signals, was blocked by flumazenil and mimicked by zolpidem, and in a computer model it bound the benzodiazepine site. ⁴³	Animal research
Dopamine pathway enzyme and brain growth factor (BDNF)	TH (tyrosine hydroxylase) and BDNF genes	After 60 minutes of inhaled alpha-pinene, TH gene activity in the midbrain was higher than in controls and movement in a maze increased. BDNF gene activity in the olfactory bulb and hippocampus was similar at 60 and 90 minutes. ¹⁶	Animal research
Serotonin, adrenaline-type and dopamine receptors	5-HT1A, beta-adrenergic and D1 receptors	In mice, the antidepressant-like effect of beta-pinene in the forced swimming test was blocked by drugs for these receptors, which points to the monoamine systems. PCPA, which blocks serotonin synthesis, did not change it. ¹⁰	Animal research
Antioxidant defence (Nrf2 and HO-1)	NFE2L2 (Nrf2), HMOX1 (HO-1)	In rat nerve-like cells under oxidative stress, alpha-pinene lowered free radicals and raised antioxidant enzymes, which the authors link to free-radical scavenging and Nrf2. In rats with alcohol stomach damage, alpha-pinene raised Nrf2 and HO-1 activity. ^{31 32}	Laboratory research
Acetylcholine-induced airway muscle contraction and possibly acetylcholinesterase	Acetylcholinesterase (suggested by the authors)	In isolated rat windpipe rings, alpha- and beta-pinene made contractions caused by acetylcholine stronger. The authors write that inhibition of the acetylcholinesterase enzyme may be involved; this was not tested directly in the abstract. ²²	Laboratory research
Microbial killing by the (+) forms	Not defined in the abstracts	Only the (+) forms of alpha- and beta-pinene were active against the bacteria and fungi tested; the (-) forms were inactive up to 20 mg/mL. Phospholipase and esterase activities were also reduced. ³³	Laboratory research
Liver metabolism to alpha-pinene oxide	Enzymes not named in the abstract	Rat and human liver microsomes and hepatocytes turned alpha-pinene into alpha-pinene oxide, with up to 4-fold higher formation in rats than in humans. ⁴⁰	Laboratory research
Effect of THC on memory in people	Not studied	Inhaled alpha-pinene did not mitigate THC-induced memory impairment in a 19-person trial, so no human mechanism for the memory idea was shown. ¹⁷	Human data

Absorption, metabolism and elimination

Absorption: By inhalation, about 60% of alpha-pinene breathed in was taken up by volunteers in exposure chambers (two studies). In an experiment with turpentine vapour in eight men, uptake was 62% for alpha-pinene and 66% for beta-pinene. By mouth, alpha-pinene was absorbed in rats and mice, with oral bioavailability of 22% to 49% in female rats and 5% to 33% in male rats depending on dose, and about 19% in mice. Human oral absorption data and blood levels after vaporizing were not given in the abstracts.
Distribution: A long half-time in poorly perfused tissues suggests a high affinity for body fat in people. In mice, inhaled alpha-pinene reached all brain regions measured and built up in brain and liver, peaking on the third day of daily inhalation. In rats and mice, both alpha-pinene and its oxide were found in mammary tissue after oral dosing.
Metabolism: Alpha-pinene is changed into verbenols (about 1% to 4% of the inhaled amount appeared in urine as cis- and trans-verbenol) and, in human and rat liver preparations, into alpha-pinene oxide.
Elimination: Less than 0.001% of the inhaled alpha-pinene left in urine unchanged and about 8% was breathed out. Most verbenols left within 20 hours of a 2-hour exposure. The last-phase blood half-lives after turpentine exposure averaged about 32 hours for alpha-pinene and 25 hours for beta-pinene. Total blood clearance was about 1.1 litres per hour per kilogram.
Bioavailability: Not established for vaporized or cannabis-flower pinene in people. The chamber studies used both (+)- and (-)-alpha-pinene; the human data are mostly for alpha-pinene or turpentine.

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12benefits and uses looked at

45studies and sources checked

Very low certaintybest evidence rating found

15lab markers tracked in trials

8 very low certainty3 not enough evidence1 evidence against

What was looked at

The 12 benefits and uses checked for Pinene (Alpha-Pinene and Beta-Pinene), each with how strong the evidence is. Tap one to open it.

Offsetting THC memory problemsEvidence against
Anxiety-like behaviour in animalsVery low certainty
SleepVery low certainty
Memory and thinking (rosemary scent and cell studies)Very low certainty
Low mood-like behaviour (mostly beta-pinene)Very low certainty
Airways, cough and bronchitisVery low certainty
Killing germs in the lab (antibacterial and antifungal)Very low certainty
Immune cells and stress hormones (cypress oil vapour)Very low certainty
Stomach lining protection (rats)Very low certainty
Cancer cell studies (not a treatment)Not enough evidence
"Pine strain" and strain-type claimsNot enough evidence
Other reported activities (review only)Not enough evidence

Lab markers tracked in the studies

Working memory and thinking tests after THCHow strong the high feels, and heart rate and blood pressureBehaviour in a raised maze (mice)Alpha-pinene in brain and liver tissue (mice)Non-REM sleep time and time to fall asleep (mice)Share of the night in deep plus REM sleep (wrist tracker)Computer memory and attention test scores (rosemary scent)Immobility in the forced swimming test (mice)Share of bronchitis patients who respondAirway muscle contraction (isolated rat windpipe)Airway reactivity and inflammation markers (mice)Lowest concentration that stops microbes growing (MIC)Natural killer cell activity and urinary stress hormonesStomach lesions and stomach pH (rats)Cancer cell migration and colony formation

What is Pinene (Alpha-Pinene and Beta-Pinene) good for?

The uses people ask about and how strong the evidence is for each. For pinene, almost all of it is from animals or cells, and the human studies are small or tested whole oils or mixtures.

Offsetting THC memory problemsEvidence againstThe one human trial found it does not. In 19 healthy adults, inhaled alpha-pinene (0.5, 5 or 15 mg) did not reduce THC memory impairment or change THC's other effects, and alpha-pinene alone matched placebo. Beta-pinene was not tested. One small trial, but nothing shows the claim is true. Anxiety-like behaviour in animalsVery low certaintyIn mice, breathing alpha-pinene or alpha-pinene-rich cypress oil vapour gave anxiolytic-like behaviour in a maze at low levels, and a more excitatory pattern at a high level. Animal research only; no human trial of pure pinene. SleepVery low certaintyIn mice, (-)-alpha-pinene by mouth lengthened non-REM sleep through the GABA-A benzodiazepine site. In people, a nine-ingredient mix (CBD plus eight terpenes, 1 mg of alpha-pinene) gave a marginal gain in one wrist-tracker measure, which cannot be credited to pinene. Memory and thinking (rosemary scent)Very low certaintyRosemary oil, rich in alpha-pinene, changed some memory scores in 144 healthy volunteers, but the follow-up linked scores to 1,8-cineole in the blood, not pinene. Alpha-pinene protected nerve-like cells from oxidative stress in a dish. Low mood-like behaviour (beta-pinene)Very low certaintyIn mice, beta-pinene reduced immobility in a forced swimming test; alpha-pinene did not. No human trial. Airways, cough and bronchitisVery low certaintyA three-terpene distillate (alpha-pinene, limonene, 1,8-cineole) beat placebo in 676 adults with acute bronchitis, but cannot be credited to alpha-pinene. In animals the results were mixed: pine oil eased airway reactivity but raised inflammation markers, and the pinenes made isolated rat windpipe muscle contract more. Killing germs in the labVery low certaintyOnly the (+) forms of the pinenes killed bacteria and fungi in test tubes, at high concentrations that also reduced mouse immune-cell survival. Beta-pinene boosted some antibiotics against Staphylococcus aureus in the lab. Not tested as a treatment in people. Immune cells and stress hormones (cypress oil vapour)Very low certaintyIn 12 healthy men, three nights of hinoki cypress oil vapour went with higher natural killer cell activity and lower urinary stress hormones than a normal day. No placebo group, and the oil has many components. Stomach lining protectionVery low certaintyIn rats given alcohol, injected alpha-pinene lessened stomach damage and raised stomach pH. No human trial. Cancer cell studies (not a treatment)Not enough evidenceIn human brain-cancer cells in a dish, alpha-pinene with the drug temozolomide reduced cell movement and colony growth more than either alone. A laboratory result only. "Pine strain" and strain-type claimsNot enough evidencePinenes are among the main cannabis terpenes, but 140 German medicinal flowers showed no link between terpene profile and sativa, indica or hybrid labels or estimated effects. No trial tested whether the pinene content of flower changes how it feels. Other reported activities (review only)Not enough evidenceA review lists many reported actions (anti-inflammatory, pain, anticonvulsant, cell protection). We read only its abstract, which gives no certainty rating, and the work behind it is mostly cells and animals.

Benefits, one by one

Each card is a research question, not a promise. Tap a goal to filter. Open a card to see the studies, doses and limits.

Offsetting THC memory problems

Evidence against

A popular claim says pinene cancels the memory and thinking problems caused by THC. The one human trial that tested it found no such effect. In 19 healthy adults, inhaled alpha-pinene alone did nothing, and adding it to THC did not reduce THC's memory impairment or change its other effects. Beta-pinene was not tested.

3 studiesVaporized pure alpha-pinene and pure THC from a hand-held vaporizer i…

Studies, doses and limits

What the trials found

In a double-blind crossover trial, 19 healthy adults (last cannabis use averaged 39 days earlier) completed six sessions. They inhaled from a hand-held vaporizer: alpha-pinene alone (15 mg), THC alone (30 mg), THC with alpha-pinene (0.5, 5 or 15 mg), or placebo air. THC alone impaired thinking and working memory. Adding alpha-pinene did not reduce the memory impairment or significantly change other effects. Alpha-pinene alone matched placebo. The authors write that the result is inconsistent with some cannabis industry claims.

Results

No reduction of THC-induced memory impairment from alpha-pinene at 0.5, 5 or 15 mg. No significant effect of alpha-pinene alone (15 mg). No numbers are given in the abstract.

Effect sizes as reported

Not given in the abstract.

Why this rating

One small, blinded, randomised crossover trial (19 healthy adults) found no benefit. A single small trial cannot rule out every dose or product, but there is no human trial that shows the claim to be true. The 2011 review and a breeding paper describe the idea as a hypothesis or possibility.

Form and dose studied

Vaporized pure alpha-pinene and pure THC from a hand-held vaporizer in a lab. This is not cannabis flower, not a cannabis oil and not beta-pinene. The authors say the pinene doses were at and above those found naturally in cannabis flower.

Who was studied

19 healthy adults who had used cannabis before, on average 39 days earlier (range 2 to 365 days).

Limits

Small; healthy volunteers; one route (inhaled); only alpha-pinene; short-term single sessions; pure compounds rather than the whole plant. It does not say what happens in daily users, in medical patients or with other terpenes.

Why researchers looked

In 2011 a review suggested that terpenes in cannabis might soften some effects of THC, and the memory idea for pinene grew from there. Researchers wanted to test it directly.

Proposed mechanism Human data

The abstracts read for this question do not describe a tested mechanism. The human trial found no effect of alpha-pinene on THC memory impairment.

A proposed mechanism does not show that the effect happens in people.

THC still caused measurable impairment of thinking and working memory in this trial. Pinene is not shown to protect anyone from THC. Do not drive or make important decisions after THC.

^{17 34 20}

Anxiety-like behaviour in animals

Very low certainty

In mice, breathing alpha-pinene vapour, or the vapour of cypress oil that is rich in it, made the animals behave as if less anxious in a maze test at low vapour levels. A higher level of the cypress oil looked more stimulating instead. No human trial of pure pinene for anxiety was found.

4 studiesInhaled pure alpha-pinene vapour, or hinoki cypress essential oil vap…

Studies, doses and limits

Results

Mice breathing alpha-pinene for 90 minutes a day showed significant anxiolytic-like behaviour that stayed constant over 5 days (elevated plus maze). With hinoki cypress oil, the lower vapour level (8 microlitres per litre of air) may give an anxiolytic-like effect, while the higher level (32) gave an excitatory-like effect. In another test, 60 minutes of alpha-pinene inhalation increased distance moved in the maze.

Effect sizes as reported

Not given in the abstracts.

Why this rating

Mouse studies from one research group, using a single behaviour test. The direction depended on the vapour level. No human trial of pure pinene.

Form and dose studied

Inhaled pure alpha-pinene vapour, or hinoki cypress essential oil vapour, in mice. Doses in people cannot be taken from these studies.

Who was studied

Mice.

Limits

Animal research. The maze test is an indirect model of anxiety. Moving more in a maze does not by itself mean less anxiety. The same oil acted differently at different levels.

Why researchers looked

Researchers tested whether alpha-pinene, the main smell molecule in some of these oils, changes behaviour in mice.

Traditional use

One abstract says pine essential oils are commonly used by inhalation. The abstracts read do not describe a documented traditional medical use for pure pinene.

Proposed mechanism Animal research

Inhaled alpha-pinene reached all brain regions measured in mice and also built up in the liver, with a peak on the third day of daily inhalation. In one study it raised activity of the gene for tyrosine hydroxylase, an enzyme in the dopamine pathway.

A proposed mechanism does not show that the effect happens in people.

Pinene is not a treatment for anxiety. Anyone with anxiety that affects daily life should talk to a doctor.

^{36 15 16 35}

Sleep

Very low certainty

In mice, a dose of (-)-alpha-pinene by mouth lengthened non-REM sleep and shortened the time to fall asleep. In people, a 125-person trial of a nine-ingredient mix (CBD plus eight terpenes including 1 mg of alpha-pinene) gave a small gain in one wrist-tracker sleep measure, but the gain cannot be credited to pinene.

2 studiesMice: (-)-alpha-pinene by mouth. People: an oral mixture of CBD and e…

Studies, doses and limits

What the trials found

No human trial of pinene alone. One double-blind crossover trial in 125 adults with insomnia gave oral CBD 300 mg plus 1 mg each of eight terpenes (linalool, myrcene, phytol, limonene, alpha-terpinene, alpha-terpineol, alpha-pinene and beta-caryophyllene) for 4 weeks. Time in slow-wave plus REM sleep rose by a mean of 1.3% (95% CI 0.1 to 2.5%) compared with placebo. Total sleep time was unchanged and no adverse events were reported.

Results

Mice: more non-REM sleep and shorter sleep latency without changing REM sleep. People: a marginal gain (P = .03) in slow-wave plus REM sleep time with a mixture.

Effect sizes as reported

Mixture trial: mean difference 1.3% of the night (standard error 0.60%; 95% CI 0.1 to 2.5%).

Why this rating

The mouse work is from one study. The only human trial tested a mixture where alpha-pinene was one of nine ingredients at 1 mg, measured sleep with a wrist tracker, and showed a small effect that was marginally significant.

Form and dose studied

Mice: (-)-alpha-pinene by mouth. People: an oral mixture of CBD and eight terpenes. Neither is a cannabis flower or an essential oil.

Who was studied

Mice; 125 adults with insomnia (mixture trial).

Limits

Animal results do not predict human sleep. In the mixture trial the effect cannot be assigned to alpha-pinene, and wrist trackers are less accurate than sleep-lab recordings.

Why researchers looked

Alpha-pinene is a major molecule of pine tree oils, which have been reported to have calming and sleep-promoting effects when inhaled. Researchers asked whether pinene taken by mouth affects sleep and how.

Proposed mechanism Animal research

In mouse brain slices, (-)-alpha-pinene strengthened the response of GABA-A receptors, and a computer model placed it at the benzodiazepine binding site. The effect on sleep was mimicked by zolpidem and blocked by flumazenil, a drug that blocks that site. This was shown in mice and in the lab, not in people.

A proposed mechanism does not show that the effect happens in people.

A drug that acts at the benzodiazepine site can cause drowsiness. Pinene was not shown to do this in people. Do not drive or use machinery if you feel drowsy.

^{43 42}

Memory and thinking (rosemary scent and cell studies)

Very low certainty

Rosemary oil, which is rich in alpha-pinene, was tested as a room scent. In 144 healthy volunteers the scent improved some memory scores and slowed another. A follow-up in 20 people linked better scores to 1,8-cineole in the blood, not to pinene. Alpha-pinene protected nerve-like cells from oxidative stress in a dish.

4 studiesRoom aroma of rosemary essential oil (a mixture) in the human studies…

Studies, doses and limits

What the trials found

In a 2003 study of 144 healthy volunteers, the aroma of rosemary oil improved overall memory quality and secondary memory but slowed the speed of memory compared with no odour. Lavender worsened working memory. In 2012, 20 volunteers in a rosemary-scented room showed better cognitive scores with higher blood levels of 1,8-cineole; the study did not measure pinene.

Results

Rosemary scent: better memory quality and secondary memory, slower memory speed. Cells: less oxidative damage with alpha-pinene.

Effect sizes as reported

Not given in the abstracts.

Why this rating

The human trials used whole rosemary oil, which is a mixture, and the second study found a link with 1,8-cineole rather than pinene. The pinene result comes from cells in a dish.

Form and dose studied

Room aroma of rosemary essential oil (a mixture) in the human studies; pure alpha-pinene on cells in the lab.

Who was studied

144 and 20 healthy volunteers; rat nerve-like cells.

Limits

Whole oil is not pinene. A smell can also change mood and expectation. The 20-person study is a correlation with no control group.

Why researchers looked

Researchers tested rosemary aroma on thinking tests. Its oil is rich in alpha-pinene and 1,8-cineole, so scientists asked which parts of the oil might act.

Proposed mechanism Laboratory research

In rat nerve-like (PC12) cells under hydrogen peroxide stress, alpha-pinene and 1,8-cineole lowered free-radical production, raised several antioxidant enzymes and reduced a cell-death signal, apparently by scavenging free radicals and turning on the Nrf2 pathway. This is a cell result only.

A proposed mechanism does not show that the effect happens in people.

No safety issues were reported in these abstracts. Essential oils can cause skin allergy when applied to skin (see Safety).

^{25 26 31 37}

Low mood-like behaviour (mostly beta-pinene)

Very low certainty

In mice, beta-pinene (and linalool) reduced immobility in a forced swimming test, a common screen for antidepressant-like activity. Alpha-pinene did not in the same test. There is no human trial.

2 studiesBeta-pinene injected into the abdomen of mice. Not an oral or inhaled…

Studies, doses and limits

Results

Beta-pinene and linalool were the two main active principles of the plant oil and showed antidepressant-like and sedative-like activity in mice. Eucalyptol, limonene and alpha-pinene did not show antidepressant-like activity.

Effect sizes as reported

Not given in the abstracts.

Why this rating

Two mouse studies from one group, using injections and a screening test that is only an indirect model of depression.

Form and dose studied

Beta-pinene injected into the abdomen of mice. Not an oral or inhaled form, and not a cannabis product.

Who was studied

Mice.

Limits

Animal research only. Mouse swimming tests do not reliably predict human antidepressant effects.

Why researchers looked

A Mexican plant used traditionally for sadness and fright, Litsea glaucescens, was analysed to find which of its compounds were active.

Traditional use

The plant is used in Mexican traditional medicine for illness of the nervous system, such as epilepsy, fright and sadness, according to the abstract. That is the plant, not pure pinene.

Proposed mechanism Animal research

In mice, the antidepressant-like effect of beta-pinene was blocked by drugs acting on serotonin 5-HT1A, beta-adrenergic and dopamine D1 receptors, suggesting the monoamine systems are involved.

A proposed mechanism does not show that the effect happens in people.

Do not stop or change a prescribed medicine for depression because of animal findings. If you feel very low or unsafe, contact a doctor or a crisis line.

^{10 9}

Airways, cough and bronchitis

Very low certainty

The strongest human result involving alpha-pinene is for a three-compound pharmaceutical distillate (alpha-pinene, limonene and 1,8-cineole) in 676 adults with acute bronchitis, which beat placebo. It cannot be credited to alpha-pinene alone. In animals and tissue, pine oil and the pinenes had mixed effects: pine oil eased airway reactivity in mice but raised some inflammation markers, and the pinenes made isolated rat windpipe muscle contract more.

3 studiesA standardized oral distillate of three terpenes (human trial); inhal…

Studies, doses and limits

What the trials found

A double-blind, placebo-controlled trial in 676 adults with acute bronchitis of recent onset compared 14 days of standardized myrtol (a distillate of mainly (+)-alpha-pinene, d-limonene and 1,8-cineole, 300 mg four times daily) with placebo, cefuroxime and ambroxol. Responder rate at the first check was 92.9% with myrtol versus 77.3% with placebo (p < 0.001), similar to cefuroxime (92.4%) and ambroxol (89.6%). The authors called it well tolerated.

Results

Myrtol (a mixture): more responders than placebo. Rat trachea: pinenes raised contractions. Mice: Scots pine oil (39.4% alpha-pinene) reduced airway hyperresponsiveness but increased several inflammatory chemicals.

Effect sizes as reported

Responder rate at visit 2: 92.9% (myrtol) versus 77.3% (placebo), p < 0.001. Non-responder rate at week 1: 5.3% versus 20.9%.

Why this rating

For pinene itself: no trial of pure alpha- or beta-pinene. The human trial tested a mixture, was from 2000, and funding is not stated in the abstract. The other evidence is from animals and isolated tissue, and points in different directions.

Form and dose studied

A standardized oral distillate of three terpenes (human trial); inhaled Scots pine oil (mice); pure alpha- and beta-pinene on isolated rat tissue.

Who was studied

676 adults with acute bronchitis; mice; rat tissue.

Limits

Cannot separate alpha-pinene from limonene and 1,8-cineole. The tissue and mouse results are not tests in people and do not agree with each other. Chamber studies in volunteers found airway discomfort with turpentine (see Safety).

Why researchers looked

Pine oil is commonly used by inhalation, so researchers tested whole oils, a mixture and the pinenes themselves in animals, airway tissue and people.

Proposed mechanism Animal research

In isolated rat windpipe rings, alpha- and beta-pinene strengthened contractions caused by acetylcholine and potassium, which is the opposite of relaxing airway muscle. The authors suggest the enzyme that breaks down acetylcholine may be involved. In mice, Scots pine oil reduced airway reactivity but raised several inflammation signals.

A proposed mechanism does not show that the effect happens in people.

Pinene vapour at high levels irritated the throat and airways of volunteers, and the pine oil mouse study urges caution in inflammation-related lung disease. People with asthma or lung disease should ask a doctor before breathing concentrated oils.

^{23 22 3}

Killing germs in the lab (antibacterial and antifungal)

Very low certainty

In test-tube work, the positive (+) forms of alpha- and beta-pinene killed bacteria and fungi, and beta-pinene boosted some antibiotics against Staphylococcus aureus in a wax-moth larvae model. The concentrations needed were high and also reduced mouse immune cell survival. None of this has been tested as a treatment for infection in people.

3 studiesPure (+) and (-) forms of alpha- and beta-pinene in culture, alone an…

Studies, doses and limits

Results

MICs of 117 to 4,150 micrograms per mL for the (+) forms (one study) and 64 to 4,096 micrograms per mL (another). (+)-Pinenes killed all Candida albicans within 60 minutes. Beta-pinene plus oxacillin cut bacterial load by 82.1% in larvae. At 250 micrograms per mL the (+) forms reduced mouse macrophage viability to 66.8% (alpha) and 57.7% (beta).

Effect sizes as reported

See results; the lab measures are MIC (the lowest concentration that stops growth).

Why this rating

Test-tube and insect-larvae work only. High concentrations. No human trial.

Form and dose studied

Pure (+) and (-) forms of alpha- and beta-pinene in culture, alone and with antibiotics.

Who was studied

Bacteria, fungi, mouse cells, wax-moth larvae.

Limits

A concentration that stops microbes in a dish says little about safe levels in the body. The mirror form matters, and commercial products often do not state which form they contain.

Why researchers looked

Researchers tested the pinenes against bacteria and fungi, alone and together with antibiotics.

Proposed mechanism Laboratory research

The positive (+) mirror forms of alpha- and beta-pinene were active and the negative (-) forms were not in one study. In another, beta-pinene lowered Staphylococcus aureus virulence factors and strengthened beta-lactam antibiotics such as oxacillin in the lab.

A proposed mechanism does not show that the effect happens in people.

Do not use pinene products on wounds or infections. See a doctor for infections.

^{33 44 13}

Immune cells and stress hormones (cypress oil vapour)

Very low certainty

In 12 healthy men who stayed three nights in a hotel room where hinoki cypress oil was vaporised, natural killer cell activity rose and urine stress hormones fell compared with a normal day. Alpha- and beta-pinene were measured in the air, but the oil has many components and there was no placebo group.

1 studiesVaporised hinoki cypress stem oil in a hotel room overnight for 3 nig…

Studies, doses and limits

What the trials found

Twelve healthy men aged 37 to 60 stayed at an urban hotel for 3 nights with hinoki cypress (Chamaecyparis obtusa) stem oil vaporised overnight. Compared with a normal working day before, natural killer cell activity and the percentages of NK and several killer-protein-carrying cells were significantly higher, T cells were lower, and urinary adrenaline and noradrenaline were lower. Alpha-pinene and beta-pinene were detected in the room air.

Results

Higher NK activity; lower urinary stress hormones; pinenes measurable in room air.

Effect sizes as reported

Not given in the abstract.

Why this rating

Twelve men, no placebo group, before-and-after in the same people, and a whole essential oil rather than pinene.

Form and dose studied

Vaporised hinoki cypress stem oil in a hotel room overnight for 3 nights.

Who was studied

12 healthy men aged 37 to 60.

Limits

No control group, tiny, and pinene is one of many components. It does not show that pinene caused the changes, or that immune changes mean better health.

Why researchers looked

The authors had earlier reported that forest trips raised higher natural killer cell activity. This study tested whether tree oil vapour (phytoncides) could be a factor.

Proposed mechanism Human data

The authors suggest that phytoncide exposure and lower stress hormone levels may partly contribute to higher natural killer cell activity. No tested mechanism is given for pinene.

A proposed mechanism does not show that the effect happens in people.

None reported in the abstract.

²¹

Stomach lining protection (rats)

Very low certainty

In rats given alcohol, alpha-pinene injected beforehand reduced stomach damage and raised stomach pH, similar to omeprazole. There is no human trial.

1 studiesAlpha-pinene injected into the abdomen of rats.

Studies, doses and limits

Results

At 50 and 100 mg/kg (injected into the abdomen), stomach lesions were fewer than in controls, similar to omeprazole 20 mg/kg; stomach pH rose.

Effect sizes as reported

Not given in the abstract.

Why this rating

One rat study using injections before an alcohol challenge.

Form and dose studied

Alpha-pinene injected into the abdomen of rats.

Who was studied

Rats.

Limits

Injection is not the way people would take it, and alcohol damage in rats is a short-term model.

Why researchers looked

Researchers tested whether alpha-pinene protects the stomach from alcohol-induced ulcers.

Proposed mechanism Animal research

Alpha-pinene raised activity of the antioxidant genes Nrf2 and HO-1 in the stomach of rats.

A proposed mechanism does not show that the effect happens in people.

Stomach pain, vomiting blood or black stools need urgent medical care, not pinene.

³²

Cancer cell studies (not a treatment)

Not enough evidence

In human brain-cancer cells in a dish, alpha-pinene combined with the chemotherapy drug temozolomide cut cell movement and colony formation more than either alone. This is a laboratory result only.

1 studiesPure alpha-pinene on cells.

Studies, doses and limits

Results

Less wound closure and fewer colonies with the combination than with either alone.

Effect sizes as reported

Not given in the abstract.

Why this rating

Two cell lines in a dish, no animal or human data in the abstract.

Form and dose studied

Pure alpha-pinene on cells.

Who was studied

Human cell lines.

Limits

Cells in a dish do not show that alpha-pinene reaches a tumour or helps a patient. This is not evidence that cannabis or pine oil treats cancer.

Why researchers looked

Many plant molecules are screened in cancer cell lines to look for ones that strengthen existing drugs.

Proposed mechanism Laboratory research

Alpha-pinene combined with temozolomide had a synergistic inhibitory effect on cell migration, soft-agar colony growth and cell death in LN229 and U87MG glioblastoma cells.

A proposed mechanism does not show that the effect happens in people.

Never replace cancer treatment with a terpene or oil.

⁷

"Pine strain" and strain-type claims

Not enough evidence

Pinene is one of the main cannabis terpenes, but strain names and sativa or indica labels do not reliably tell you what is in the flower, and a terpene profile does not tell you what the flower will feel like. A 2025 analysis of 140 German medicinal flowers found no link between terpene profile and sativa, indica or hybrid labels or estimated effects.

3 studiesDried cannabis flower, measured by laboratory methods.

Studies, doses and limits

What the trials found

No trial tested whether the pinene content of cannabis flower changes how it feels.

Results

Chemistry papers show pinenes are among the main cannabis terpenes. In 33 samples, alpha-pinene was 5.8% of total terpenes in CBD-dominant and 4.9% in THC-dominant samples, and beta-pinene 5.0% and 5.8%. Ten plants of one strain grown together varied by about 20% in terpenes above 20 micrograms per gram. In 140 German flowers there was no statistical correlation between terpene profiles and genetic type.

Why this rating

The chemistry is well measured, but nothing tests the effect claim. The labels have been shown not to predict terpene profiles.

Form and dose studied

Dried cannabis flower, measured by laboratory methods.

Who was studied

Cannabis flower samples.

Limits

Percent of total terpenes is not the same as milligrams per gram. Lab methods and samples differ. Our lab numbers on the Overview tab show how much the same strain name varies.

Why researchers looked

Strains are often marketed by smell, such as "pine", and terpenes are linked to effects.

Proposed mechanism Human data

No tested mechanism links the pinene content of a cannabis flower to its effects. The only human trial found no effect of added alpha-pinene on THC effects (see the mind tab).

A proposed mechanism does not show that the effect happens in people.

A pinene figure on a label tells you nothing about safety or effect.

^{4 8 12}

Other reported activities (review only)

Not enough evidence

A review lists many reported actions of the pinenes: anti-inflammatory, pain-relieving, anticonvulsant, protection of cells from oxidative stress, effects on pancreatitis and tooth-pulp pain, and more. The abstract gives no certainty rating and the work behind it is mostly from cells and animals.

1 studiesAlpha- and beta-pinene (review).

Studies, doses and limits

What the trials found

The abstract does not describe human trials. We read only the abstract.

Results

A list of reported activities, not trial results.

Effect sizes as reported

Not given.

Why this rating

We read only the abstract of a narrative review of mostly preclinical research. We cannot say which claims rest on human data.

Form and dose studied

Alpha- and beta-pinene (review).

Who was studied

Mostly cells and animals.

Limits

No certainty assessment is given.

Why researchers looked

Reviewers collected scattered findings on the two pinenes.

Proposed mechanism Animal research

The review summarises cytogenetic, gastroprotective, anxiolytic, cytoprotective, anticonvulsant and neuroprotective effects, with effects against hydrogen-peroxide stress, pancreatitis, stress-stimulated fever and pulpal pain. It also discusses bioavailability and clinical applications.

A proposed mechanism does not show that the effect happens in people.

None given in the abstract.

³⁵

Connections to the rest of health

How Pinene (Alpha-Pinene and Beta-Pinene) connects to the rest of our health knowledge graph. Every link says what kind of connection it is. A safety link (for example to the liver) is a warning, not a benefit. A study that merely mentions a topic never creates a benefit link.

Explore every connection: goals, body systems, organs, symptoms, genes

Kind of connection Connected to Search

B. Body systems

Connected to	Kind of connection	Evidence status	Context	Studies
Respiratory system	Adverse effect or risk	Low certainty	Throat and airway irritation in volunteers at 450 mg per cubic metre; airway resistance rose after turpentine. Preparation or compound: Inhaled pinene vapour at high levels	^{5 6}
Nervous system		Low certainty	No reduction of THC memory impairment in 19 healthy adults. Preparation or compound: Inhaled alpha-pinene with THC	¹⁷
Nervous System	Biological association	Evidence against	Studied for: Offsetting THC memory problems Studied for: Anxiety-like behaviour in animals	^{17 34 20 36 15 16 35 25 26 31 37 7 4 8 12}
Sleep Recovery	Biological association	Very low certainty	Studied for: Sleep	^{43 42}
Stress Mood	Biological association	Very low certainty	Studied for: Low mood-like behaviour (mostly beta-pinene)	^{10 9}
Respiratory System	Biological association	Very low certainty	Studied for: Airways, cough and bronchitis	^{23 22 3}
Immune System	Biological association	Very low certainty	Studied for: Killing germs in the lab (antibacterial and antifungal) Studied for: Immune cells and stress hormones (cypress oil vapour)	^{33 44 13 21 35}
Digestive System	Biological association	Very low certainty	Studied for: Stomach lining protection (rats)	³²

C. Body parts and organs

Connected to	Kind of connection	Evidence status	Context	Studies
Skin	Adverse effect or risk	Low certainty	17 of 22 turpentine-allergic patients reacted to alpha-pinene on patch testing; 8 of 24 pottery workers. Preparation or compound: Turpentine and pinene-rich oils	^{2 18 14}

I. Biomarkers

Connected to	Kind of connection	Evidence status	Context	Studies
Working memory and thinking tests after THC	Biological association	Evidence against	Measured in trials: no change. A lab change is not the same as a health benefit.	¹⁷
How strong the high feels, and heart rate and blood pressure	Biological association	Evidence against	Measured in trials: no change. A lab change is not the same as a health benefit.	¹⁷
Behaviour in a raised maze (mice)	Biological association	Very low certainty	Measured in trials: mixed. A lab change is not the same as a health benefit.	^{36 15 16}
Alpha-pinene in brain and liver tissue (mice)	Biological association	Very low certainty	Measured in trials: present. A lab change is not the same as a health benefit.	^{36 15}
Non-REM sleep time and time to fall asleep (mice)	Biological association	Very low certainty	Measured in trials: increased. A lab change is not the same as a health benefit.	⁴³
Share of the night in deep plus REM sleep (wrist tracker)	Biological association	Very low certainty	Measured in trials: increased. A lab change is not the same as a health benefit.	⁴²
Computer memory and attention test scores (rosemary scent)	Biological association	Very low certainty	Measured in trials: mixed. A lab change is not the same as a health benefit.	^{25 26}
Immobility in the forced swimming test (mice)	Biological association	Very low certainty	Measured in trials: lowered. A lab change is not the same as a health benefit.	^{9 10}
Share of bronchitis patients who respond	Biological association	Very low certainty	Measured in trials: increased. A lab change is not the same as a health benefit.	²³
Airway muscle contraction (isolated rat windpipe)	Biological association	Very low certainty	Measured in trials: increased. A lab change is not the same as a health benefit.	²²
Airway reactivity and inflammation markers (mice)	Biological association	Very low certainty	Measured in trials: mixed. A lab change is not the same as a health benefit.	³
Lowest concentration that stops microbes growing (MIC)	Biological association	Very low certainty	Measured in trials: lowered. A lab change is not the same as a health benefit.	^{33 44 13}
Natural killer cell activity and urinary stress hormones	Biological association	Very low certainty	Measured in trials: increased. A lab change is not the same as a health benefit.	²¹
Stomach lesions and stomach pH (rats)	Biological association	Very low certainty	Measured in trials: lowered. A lab change is not the same as a health benefit.	³²
Cancer cell migration and colony formation	Biological association	Not enough evidence	Measured in trials: lowered. A lab change is not the same as a health benefit.	⁷

L. Active compounds

Connected to	Kind of connection	Evidence status	Context	Studies
(+)-alpha-Pinene	Biological association	Mixed findings	Active against microbes in the lab; the form made by the Finola hemp enzymes. The abstract of the human trial of alpha-pinene with THC does not state which mirror form was used. Preparation or compound: Rosemary oil (13.5% to 37.7% as (+)-alpha-pinene), Scots pine oil (39.4% alpha-pinene), cannabis resin	^{33 1 37 17}
(-)-alpha-Pinene	Biological association	Animal	Lengthened non-REM sleep in mice through the GABA-A benzodiazepine site; inactive against microbes in one lab study. Preparation or compound: Not examined in the abstracts read	^{43 33}
Beta-pinene	Biological association	Animal	Antidepressant-like in mouse swimming tests (alpha-pinene was not); strengthened beta-lactam antibiotics against S. aureus in the lab. Mirror forms differ in toxicity in a brine shrimp screen. Preparation or compound: Cannabis resin; turpentine; room air with cypress oil	^{10 9 44 13}
Alpha-pinene oxide	Biological association	In vitro	Formed in rat and human liver preparations; mutagenic in bacteria at 25 micrograms per plate and above. Preparation or compound: Not a plant compound in the sources read; formed in the body and in the lab from alpha-pinene	^{40 41}
Verbenols (cis- and trans-verbenol)	Biological association	Human	About 1% to 4% of inhaled alpha-pinene was passed in urine as cis- and trans-verbenol, mostly within 20 hours. Preparation or compound: Not a plant compound in the sources read	¹⁹
3-Carene (delta-3-carene)	Biological association	Human	Its hydroperoxides were the main culprit in Scandinavian turpentine allergy. Preparation or compound: Some turpentine	^{14 6}
1,8-Cineole (eucalyptol)	Biological association	Mixed findings	The compound whose blood level tracked cognitive scores after rosemary aroma, and protected nerve-like cells with alpha-pinene in a dish. Preparation or compound: Rosemary oil (16.1% to 29.3% in six origins)	^{26 31 37}
d-Limonene	Biological association	Mixed findings	A separate cannabis terpene with its own profile page here; part of the three-terpene bronchitis distillate. Preparation or compound: Citrus peel oils; cannabis; with alpha-pinene in the bronchitis distillate	²³

Each connection is stored with the ingredient, the connected record, the kind of connection, the evidence status, the supporting studies, the preparation or compound involved, and a review date (last reviewed October 25, 2026).

Evidence at a glance: what changed in 7 lab markers

Tap a number to show only those markers. Counts are markers, not people.

Other43

The evidence

The research centre for Pinene (Alpha-Pinene and Beta-Pinene). Every record here was checked against PubMed or the publisher's page; we never list a study we have not verified.

Evidence by outcome

Bars show how many studies we found for each outcome, not how well it works. People are added up across trials only (reviews are not added, to avoid double counting).

How we rate evidence

High certainty

Several well-run trials or reviews agree; further research is unlikely to change the picture much.

Moderate certainty

Good trials point the same way, but some doubt remains.

Low certainty

Small, short or inconsistent trials. The finding could change with better research.

Very low certainty

Very little or very weak human data. Treat as a possibility only.

Mixed findings

Trials or reviews disagree with each other.

Not enough evidence

Not enough human research to say either way.

Evidence against

Good research found no benefit, or found harm.

We take the reviewers' own risk-of-bias and GRADE statements where they exist, and otherwise judge from study size, design, consistency, directness and whether a product matches what is sold. Many publications do not make evidence high. Method note: We searched PubMed and read abstracts of about 40 sources, read the FDA flavouring list through the Electronic Code of Federal Regulations, read PubChem for identity and hazard classifications, and ran the PubChem identity check. Only abstracts were read for papers. Certainty ratings are the compiler's own reading. EFSA, JECFA, IFRA, EU cosmetics rules, LiverTox and LactMed were not read. The lab numbers are from public regulator datasets and published papers, not our own testing.

Study library: every study we checked 45

Design Research type Year from Search

Study	Design	People	Product, dose, length	Result
Booth 2017 Terpene synthases from Cannabis sativa. PLoS One · PMID 28355238 · DOI	Lab analysis plant	Trichomes of the hemp variety Finola	Cannabis terpene-making enzymes (gene and enzyme study) Resin glands (trichomes)	Nine terpene synthase genes were found. Their products include beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene and alpha-humulene, which make up most of the Finola resin terpenes. Cannabis enzymes make (+)-alpha-pinene (in this variety) Methods, limits and funding Outcomes: Which terpenes the plant's enzymes make Limitations: One hemp variety. Beta-pinene is not named among the products in the abstract. Funding and conflicts: Not stated in the abstract. Read: abstract
Cachão 1986 Allergy to oil of turpentine in Portugal. Contact Dermatitis · PMID 3720296 · DOI	Observational	22 Patients in Portugal allergic to oil of turpentine	Patch tests with six terpenes Isolated compounds Patch test	Turpentine allergy was common in Portugal even though the turpentine contained no delta-3-carene. Of 22 patients, 17 were allergic to alpha-pinene and 15 to dipentene (12 to both); 4 reacted to delta-3-carene, 3 to alpha-terpineol and 2 to beta-pinene. 17 of 22 turpentine-allergic patients reacted to alpha-pinene; 2 to beta-pinene Methods, limits and funding Outcomes: Positive patch-test reactions Limitations: Patients selected for turpentine allergy, so this is not a rate for the general public. A 1986 report with no oxidation details in the abstract. Funding and conflicts: Not stated in the abstract. Read: abstract
Csikós 2022 Pinus sylvestris L. and Syzygium aromaticum (L.) Merr. & L. M. Perry Essential Oils Inhibit Endotoxin-Induced Airway Hyperreactivity despite Aggravated Inflammatory Mechanisms in Mice. Molecules · PMID 35744988 · DOI	Animal research mouse	Mice with endotoxin-induced lung inflammation	Scots pine (Pinus sylvestris) essential oil and clove oil inhaled Essential oil Three inhalations over 24 hours 24 hours · vs Control	Alpha-pinene was 39.4% of the commercial Scots pine oil. Both oils reduced airway hyperresponsiveness, but MPO activity and several inflammatory chemicals (IL-1 beta, KC, MCP-1, MIP-2, TNF-alpha) were increased. The authors suggest caution in inflammation-related lung disease. Pine oil eased airway reactivity but raised some inflammation markers in mice Methods, limits and funding Outcomes: Airway reactivity, lung inflammation markers Limitations: Whole oils in mice; not pure alpha-pinene. Funding and conflicts: Hungarian research programme funding listed in the record. Read: abstract
Dei Cas 2021 Characterization of chemotype-dependent terpenoids profile in cannabis by headspace gas-chromatography coupled to time-of-flight mass spectrometry. J Pharm Biomed Anal · PMID 34111731 · DOI	Lab analysis plant	33 Flower from 20 THC-dominant and 13 CBD-dominant cannabis samples	Headspace gas chromatography with mass detection Flower	Alpha-pinene was 5.8% (CBD-dominant) versus 4.9% (THC-dominant) of total terpene content, and beta-pinene 5.0% versus 5.8%. The method detected down to 5 ppm. Both pinenes are among the main cannabis terpenes in these samples; the differences between chemotypes are small Methods, limits and funding Outcomes: Terpene profile by chemotype Limitations: A small sample set from one method. Percent of total terpenes is not the same as amount per gram. Funding and conflicts: Not stated in the abstract. Read: abstract
Falk 1990 Uptake, distribution and elimination of alpha-pinene in man after exposure by inhalation. Scand J Work Environ Health · PMID 2255878 · DOI	Pharmacokinetic study	Human volunteers in an exposure chamber	Inhaled (+)- and (-)-alpha-pinene vapour Isolated compound About 10, 225 and 450 mg per cubic metre of air; (-) form at 450 2 hours at light exercise, four occasions	About 60% of the inhaled amount was absorbed at the higher exposures. Blood clearance was high. Less than 0.001% left unchanged in urine and about 8% in exhaled air. A long half-time suggests a high affinity for fat tissue. Five subjects reported irritation with a significant exposure-response relationship. Lung function 20 minutes after exposure did not change. Well absorbed by inhalation; irritation at chamber exposures; no short-term change in lung function Methods, limits and funding Outcomes: Uptake, blood levels, elimination, irritation, lung function Limitations: Healthy volunteers, two-hour exposures at air levels far above ordinary indoor air. Number of subjects not given in the abstract. Funding and conflicts: Not stated in the abstract. Read: abstract
Filipsson 1996 Short term inhalation exposure to turpentine: toxicokinetics and acute effects in men. Occup Environ Med · PMID 8777445 · DOI	Pharmacokinetic study	8 Eight male volunteers	Inhaled turpentine vapour (a mixture of monoterpenes: alpha-pinene, beta-pinene, 3-carene) Vapour 450 mg per cubic metre 2 hours at 50 W · vs Control concentrations	Relative uptake was 62% for alpha-pinene and 66% for beta-pinene. Blood half-lives of the last phase were about 32 hours (alpha-pinene) and 25 hours (beta-pinene). Volunteers reported throat and airway discomfort, and airway resistance rose after the exposure, a result that differed significantly from 3-carene exposure. Airway discomfort and a rise in airway resistance after a 450 mg per cubic metre turpentine exposure Methods, limits and funding Outcomes: Uptake, blood clearance, discomfort, airway resistance Limitations: Eight men, a mixture rather than pure pinene, high chamber exposure. Funding and conflicts: Not stated in the abstract. Read: abstract
Gautam 2024 Comparative analysis of α-pinene alone and combined with temozolomide in human glioblastoma cells. Nat Prod Res · PMID 37665021 · DOI	Laboratory research cell	Human glioblastoma cell lines (LN229, U87MG)	Alpha-pinene alone and with temozolomide Isolated compound · vs Temozolomide alone	In combination, alpha-pinene and temozolomide had a synergistic inhibitory effect on the cancer cells, with reduced wound closure and fewer colonies than either compound alone. Lab effect on brain cancer cells in a dish Methods, limits and funding Outcomes: Cell migration, colony formation, cell death Limitations: Cells in a dish only; no animal or human data in the abstract. Not evidence that alpha-pinene treats cancer. Funding and conflicts: Not stated in the abstract. Read: abstract
Giovannoni 2023 Determination of variability of terpenes and terpenoids in Cannabis sativa by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. J Chromatogr A · PMID 36463647 · DOI	Lab analysis plant	20 Flower of two cannabis strains, ten plants of each grown together	Solid-phase microextraction with gas chromatography Flower	Alpha-pinene and beta-pinene were among the high-amount terpenes in both strains. Variability between ten plants of one strain grown together and harvested together was about 20% for terpenes above 20 micrograms per gram. Pinenes were high-amount terpenes in both strains; plant-to-plant variation about 20% Methods, limits and funding Outcomes: Terpene amounts and how much they vary Limitations: Two strains only; the abstract gives no pinene amounts. Funding and conflicts: Not stated in the abstract. Read: abstract
Guzmán-Gutiérrez 2012 Antidepressant activity of Litsea glaucescens essential oil: identification of β-pinene and linalool as active principles. J Ethnopharmacol · PMID 22867633 · DOI	Animal research mouse	Mice given Litsea glaucescens essential oil and its main compounds	Essential oil and beta-pinene, linalool, eucalyptol, limonene and alpha-pinene injected into the abdomen Essential oil and isolated compounds Oil 100 and 300 mg/kg Single dose · vs Control	The oil had antidepressant-like activity. Beta-pinene and linalool were its active principles, with antidepressant-like and sedative-like activity. Eucalyptol, limonene and alpha-pinene did not show antidepressant-like activity. Beta-pinene, but not alpha-pinene, was active in the antidepressant-like test in mice Methods, limits and funding Outcomes: Forced swimming, open field, elevated plus maze, rotarod Limitations: Mice, injected. A mouse test only; no human data. Funding and conflicts: Not stated in the abstract. Read: abstract
Guzmán-Gutiérrez 2015 Linalool and β-pinene exert their antidepressant-like activity through the monoaminergic pathway. Life Sci · PMID 25771248 · DOI	Animal research mouse	Mice in the forced swimming test	Beta-pinene and linalool injected into the abdomen Isolated compounds Single dose with blocking drugs · vs Control	The antidepressant-like effect of beta-pinene was blocked by drugs acting on serotonin 5-HT1A, beta-adrenergic and dopamine D1 receptors, suggesting the monoamine systems are involved. Antidepressant-like effect of beta-pinene in mice; mechanism studied Methods, limits and funding Outcomes: Immobility time in the forced swimming test (antidepressant-like effect) Limitations: A mouse behaviour test that is only an indirect model of depression. Not tested in people. Funding and conflicts: Not stated in the abstract. Read: abstract
Güzel 2015 A lethal danger in the home: turpentine poisoning. Turk J Pediatr · PMID 26690600	Case series	1 A 9-year-old boy who accidentally drank a turpentine oil hair-care preparation	Turpentine oil (swallowed) Oil Single ingestion	The report lists signs that may develop in turpentine poisoning: haematuria, kidney failure, loss of vision, chest pain, vomiting, severe coughing, bleeding, low blood pressure and death. The boy had slow heart rate and low blood pressure that was corrected over two and a half days in hospital; laboratory tests were normal. Swallowed turpentine can cause serious harm; this child recovered after hospital care Methods, limits and funding Outcomes: Clinical course Limitations: A single case report. Turpentine is a mixture that contains pinenes; the report does not isolate pinene. Funding and conflicts: Not stated in the abstract. Read: abstract
Herwig 2025 Classification of Cannabis Strains Based on their Chemical Fingerprint-A Broad Analysis of Chemovars in the German Market. Cannabis Cannabinoid Res · PMID 39137353 · DOI	Lab analysis plant	140 140 medicinal cannabis flowers on the German market	Gas chromatography-mass spectrometry of terpene profiles Flower	There was no statistical correlation between terpene profiles and genetic type labels. Profiles within sativa, indica and hybrid groups were heterogeneous, and the authors say there is no relation between terpenes and the estimated pharmacological effect. Strain-type labels do not predict terpene profiles Methods, limits and funding Outcomes: Whether terpene profiles match sativa, indica or hybrid labels or estimated effects Limitations: Medicinal flowers on one market; mostly THC-dominant; the abstract does not give pinene-specific numbers. Funding and conflicts: Not stated in the abstract. Read: abstract
Hoosen 2026 Stereoisomeric interactions of α- and β-pinene with essential oil compounds. Fitoterapia · PMID 41253204 · DOI	Laboratory research microbe and brine shrimp	Microbes and brine shrimp in the lab	Alpha- and beta-pinene mirror forms alone and with 14 essential oil compounds Isolated compounds MICs 0.25 to 4.00 mg/mL 48 hours for shrimp · vs Single compounds	Alpha- and beta-pinene mirror forms had comparable activity alone. S-(-)-beta-pinene was more toxic to brine shrimp (90.82% deaths) than R-(+)-beta-pinene (30.75%); both alpha-pinene forms were non-toxic in that test. Mirror forms behave differently in combinations and in toxicity Methods, limits and funding Outcomes: Antimicrobial, anti-quorum-sensing and toxicity profiles Limitations: Lab and brine-shrimp work. Brine-shrimp lethality is a rough toxicity screen. Funding and conflicts: Not stated in the abstract. Read: abstract
Karlberg 2021 One hundred years of allergic contact dermatitis due to oxidized terpenes: What we can learn from old research on turpentine allergy. Contact Dermatitis · PMID 34453446 · DOI	Review	Review of terpene allergy from turpentine to modern products	Oxidised monoterpenes (including turpentine)	Turpentine caused occupational contact dermatitis in painters from the early 20th century. The researchers concluded that the main culprit in Scandinavian turpentine was delta-3-carene hydroperoxides. Patch-test studies showed monoterpene hydroperoxides are a common cause of contact allergy in the population. Oxidised monoterpenes are a recognised cause of contact allergy Methods, limits and funding Outcomes: Contact allergy from oxidised terpenes Limitations: A history review. The abstract focuses on delta-3-carene, limonene and linalool rather than pinene. Funding and conflicts: Not stated in the abstract. Read: abstract
Kasuya 2015 Intracerebral Distribution of a-Pinene and the Anxiolytic-like Effect in Mice Following Inhaled Administration of Essential Oil from Chamaecyparis obtuse. Nat Prod Commun · PMID 26434146	Animal research mouse	Mice breathing hinoki cypress (Chamaecyparis obtusa) essential oil vapour	Hinoki cypress essential oil (alpha-pinene is its main volatile) Essential oil 8 and 32 microlitres per litre of air · vs Control air	Alpha-pinene reached all brain regions measured. The lower vapour level (8 microlitres per litre) may produce an anxiolytic-like effect, and the higher level (32) an excitatory-like effect. Anxiolytic-like at a low vapour level, excitatory-like at a high level, in mice Methods, limits and funding Outcomes: Alpha-pinene in brain regions; emotional behaviour Limitations: Whole essential oil, not pure alpha-pinene. Animals only. Funding and conflicts: Not stated in the abstract. Read: abstract
Kasuya 2015 Expression of BDNF and TH mRNA in the brain following inhaled administration of α-pinene. Phytother Res · PMID 25230317 · DOI	Animal research mouse	Mice breathing alpha-pinene for 60 or 90 minutes	Inhaled alpha-pinene Isolated compound 10 microlitres per litre of air 60 or 90 minutes · vs Water	60 minutes of inhalation increased total distance travelled in the maze, brain alpha-pinene was higher at 60 than at 90 minutes, and TH gene activity in the midbrain was higher than in controls. Raised movement in the maze and TH gene activity in mice Methods, limits and funding Outcomes: Elevated plus maze; brain levels; BDNF and TH gene activity Limitations: Mice only. The authors link the findings to an anxiolytic-like effect but the measure used (distance moved) is indirect. Funding and conflicts: Not stated in the abstract. Read: abstract
Kumar 2025 The Individual and Interactive Effects of Alpha-Pinene and Delta-9-Tetrahydrocannabinol in Healthy Adults. Med Cannabis Cannabinoids · PMID 40734690 · DOI	Controlled trial	19 Healthy adults whose last cannabis use averaged 39 days earlier (range 2 to 365)	Vaporized alpha-pinene alone, THC alone, or THC plus alpha-pinene (pure compounds) Isolated compounds Alpha-pinene 15 mg alone; THC 30 mg alone; THC 30 mg plus alpha-pinene 0.5, 5 or 15 mg Six outpatient sessions, outcomes up to 6 hours · vs Placebo (ambient air)	15 mg alpha-pinene alone produced no significant effects compared with placebo. 30 mg THC alone impaired cognitive performance and working memory. Adding alpha-pinene did not reduce THC-related memory impairment or significantly change the other measured effects. No effect of alpha-pinene on THC memory impairment or other effects Methods, limits and funding Outcomes: Subjective drug effects, cognitive and movement tests, working memory, vital signs Limitations: Small (19), healthy adults, one route (hand-held vaporizer), pure compounds rather than cannabis flower, short term. The authors say the doses were at and above those found naturally in cannabis flower and that the result is inconsistent with some industry claims. Only alpha-pinene was tested; beta-pinene was not. Funding and conflicts: Not stated in the abstract. Read: abstract
Lear 1996 Transient re-emergence of oil of turpentine allergy in the pottery industry. Contact Dermatitis · PMID 8930478 · DOI	Case series	24 Hand dermatitis in pottery workers doing ceramic decoration	Oil of turpentine (Indonesian) and its components by patch test Oil and isolated compounds Patch test	Of 24 workers, 14 were sensitive to Indonesian turpentine, 8 to alpha-pinene, 4 to delta-3-carene and 2 to turpentine peroxides. The authors call alpha-pinene an unusual allergen that appeared to be the most common in their area, and the problem eased when they went back to Portuguese turpentine. Alpha-pinene was a common allergen in these workers, in the absence of reactions to peroxides Methods, limits and funding Outcomes: Positive patch-test reactions Limitations: 24 workers in one occupation, 1996. A case series, not a population rate. Funding and conflicts: Not stated in the abstract. Read: abstract
Levin 1992 Renal elimination of verbenols in man following experimental alpha-pinene inhalation exposure. Int Arch Occup Environ Health · PMID 1587632 · DOI	Pharmacokinetic study	Human volunteers in an exposure chamber	Inhaled (+)- and (-)-alpha-pinene Isolated compound About 10, 225 and 450 mg per cubic metre of air 2 hours	Pulmonary uptake was about 60% and about 8% was exhaled unchanged. About 1% to 4% of the total uptake left in urine as cis- and trans-verbenol, mostly within 20 hours. Unchanged alpha-pinene in urine was under 0.001%. Verbenols in urine show how the body breaks alpha-pinene down Methods, limits and funding Outcomes: Urine verbenols (breakdown products) Limitations: Chamber study with healthy volunteers; the abstract does not give the number of subjects. Funding and conflicts: Not stated in the abstract. Read: abstract
Lewis 2018 Pharmacological Foundations of Cannabis Chemovars. Planta Med · PMID 29161743 · DOI	Lab analysis plant	Cannabis breeding programme and chemovars	Cannabis chemovars analysed for cannabinoids and terpenoids Flower	The authors describe chemovars highly potent in single terpenes, including pinene, and say specific chemovars may produce effects such as analgesia and fewer THC side effects, including short-term memory impairment. Breeding and marketing-style description; no controlled test of effects in the abstract Methods, limits and funding Outcomes: Terpenoid content of bred chemovars, scent, taste and subjective effect data Limitations: The abstract states possibilities rather than tested results, and describes the authors' own breeding programme. Funding and links to a seed or product business are not stated in the abstract. Funding and conflicts: Not stated in the abstract. Read: abstract
Li 2009 Effect of phytoncide from trees on human natural killer cell function. Int J Immunopathol Pharmacol · PMID 20074458 · DOI	Clinical trial	12 Healthy men aged 37 to 60 staying 3 nights in an urban hotel	Hinoki cypress (Chamaecyparis obtusa) stem oil vapour (phytoncides) Essential oil Vaporised with a humidifier overnight 3 nights · vs The same people on a normal working day (before)	Exposure increased NK activity and the percentage of NK and several killer-protein-carrying cells and lowered urinary adrenaline and noradrenaline. Alpha-pinene and beta-pinene were detected in the room air. Higher NK-cell activity and lower stress hormones in 12 men Methods, limits and funding Outcomes: Natural killer cell activity, immune cell counts, urine stress hormones Limitations: No placebo group (before-and-after in the same men); whole cypress oil, so pinene is not shown to be the cause. Very small. Funding and conflicts: Not stated in the abstract. Read: abstract
Lima 2010 The essential oil of Eucalyptus tereticornis, and its constituents alpha- and beta-pinene, potentiate acetylcholine-induced contractions in isolated rat trachea. Fitoterapia · PMID 20302920 · DOI	Laboratory research rat tissue	Isolated rat windpipe (trachea) rings	Eucalyptus tereticornis oil and its alpha- and beta-pinene Essential oil and isolated compounds · vs Acetylcholine and potassium contractions	Alpha- and beta-pinene potentiated contractions caused by acetylcholine and by potassium, whereas the whole oil had a muscle-relaxing effect. The authors suggest inhibition of the acetylcholinesterase enzyme may be involved. The pinenes made rat airway muscle contract more in the dish, not less Methods, limits and funding Outcomes: Contraction of airway muscle Limitations: Isolated rat tissue; says nothing about breathing in people. Funding and conflicts: Not stated in the abstract. Read: abstract
Matthys 2000 Efficacy and tolerability of myrtol standardized in acute bronchitis. A multi-centre, randomised, double-blind, placebo-controlled parallel group clinical trial vs. cefuroxime and ambroxol. Arzneimittelforschung · PMID 10994153 · DOI	Controlled trial	676 Adults with acute bronchitis of recent onset (within 5 days)	Standardized myrtol: a distillate of mainly (+)-alpha-pinene, d-limonene and 1,8-cineole Distillate (mixture) 300 mg four times daily 14 days · vs Placebo, cefuroxime, ambroxol	Responder rate at visit 2 was 92.9% for myrtol versus 77.3% for placebo (p < 0.001), similar to cefuroxime (92.4%) and ambroxol (89.6%). Non-responder rates at week 1 were 5.3% versus 20.9% on placebo. The treatments were well tolerated. The three-terpene distillate beat placebo for acute bronchitis Methods, limits and funding Outcomes: Responder rate, signs and symptoms, FEV1, safety Limitations: A three-compound mixture, so the result cannot be credited to alpha-pinene. A 2000 trial of a standardized pharmaceutical distillate; funding not stated in the abstract. Funding and conflicts: Not stated in the abstract. Read: abstract
Meehan-Atrash 2019 Aerosol Gas-Phase Components from Cannabis E-Cigarettes and Dabbing: Mechanistic Insight and Quantitative Risk Analysis. ACS Omega · PMID 31592479 · DOI	Lab analysis n/a	Laboratory vaporizing and dabbing of cannabis extracts	Heated THC with and without added terpenes (a terpene mixture) Extracts · vs THC alone	Users may be exposed to degradants such as methacrolein, benzene and methyl vinyl ketone. THC alone and mixed with terpenes gave similar products, and added terpenes gave higher levels of gas-phase products than THC alone. Added terpenes raised breakdown products in the lab test Methods, limits and funding Outcomes: Gas-phase breakdown products Limitations: A laboratory test with a terpene mixture; pinene was not tested on its own in the abstract. Funding and conflicts: Funded in part by a US NIEHS grant (listed in the record). Read: abstract
Moss 2003 Aromas of rosemary and lavender essential oils differentially affect cognition and mood in healthy adults. Int J Neurosci · PMID 12690999 · DOI	Controlled trial	144 Healthy volunteers	Aroma of rosemary or lavender essential oil in a test cubicle Essential oil Ambient aroma During testing · vs No odour	Rosemary improved overall memory quality and secondary memory but impaired speed of memory compared with controls. Lavender worsened working memory and slowed reaction times. Mixed effects on memory for rosemary aroma Methods, limits and funding Outcomes: Computerized cognitive battery and mood Limitations: Whole rosemary oil, not alpha-pinene alone (rosemary oils contain alpha-pinene and 1,8-cineole). A 2003 study; the original abstract says participants were not told the aim. Funding and conflicts: Not stated in the abstract. Read: abstract
Moss 2012 Plasma 1,8-cineole correlates with cognitive performance following exposure to rosemary essential oil aroma. Ther Adv Psychopharmacol · PMID 23983963 · DOI	Observational	20 Healthy volunteers	Aroma of rosemary essential oil in a cubicle Essential oil Diffused aroma One session	Performance on cognitive tasks was significantly related to the blood level of absorbed 1,8-cineole, with better performance at higher levels. The study looked at 1,8-cineole, not alpha-pinene. Cineole level, not pinene, tracked with scores Methods, limits and funding Outcomes: Blood level of 1,8-cineole against cognitive task scores and mood Limitations: Correlation in 20 people with no control group. It does not show that the aroma caused the change, and it did not measure pinene. Funding and conflicts: Not stated in the abstract. Read: abstract
National Center for Biotechnology Information 2026 PubChem GHS classification summary for alpha-pinene (CID 6654) PubChem PUG-View API (retrieved 2026-10-25) · Source	Authoritative guidance n/a	n/a	n/a	Of 2,036 company reports, 97.6% notified flammable liquid and vapour, 83.2% skin irritation, 66.1% may cause an allergic skin reaction, 74.1% may be fatal if swallowed and enters airways, 13.7% harmful if swallowed, 23.3% serious eye damage, and 55.7% very toxic to aquatic life with long-lasting effects; 1.2% of reports said it did not meet hazard criteria. Company notifications, not a regulator's ruling Methods, limits and funding Outcomes: Hazard classes notified by companies Limitations: Notifications vary with purity and additives. They are not a ruling by a regulator. Funding and conflicts: n/a Read: full-text
National Center for Biotechnology Information 2026 PubChem GHS classification summary for beta-pinene (CID 14896) PubChem PUG-View API (retrieved 2026-10-25) · Source	Authoritative guidance n/a	n/a	n/a	Of 2,276 company reports, 99.7% notified flammable liquid and vapour and may be fatal if swallowed and enters airways, 97.5% may cause an allergic skin reaction, 81.9% skin irritation, and 30.2% very toxic to aquatic life with long-lasting effects. A single notification (33 reports) listed toxic if inhaled and respiratory irritation. Company notifications, not a regulator's ruling Methods, limits and funding Outcomes: Hazard classes notified by companies Limitations: Notifications vary with purity and additives. They are not a ruling by a regulator. Funding and conflicts: n/a Read: full-text
National Center for Biotechnology Information 2026 PubChem compound record for alpha-pinene (CID 6654), with the (+) form CID 82227 and the (-) form CID 440968 PubChem REST API (retrieved 2026-10-25) · Source	Authoritative guidance n/a	n/a	n/a	CID 6654: 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, formula C10H16, molecular weight 136.23; CAS 80-56-8 in the synonym list. CID 82227 is (1R,5R) (the (+) form) and CID 440968 is (1S,5S) (the (-) form). Identity confirmed Methods, limits and funding Outcomes: Chemical identity Limitations: Chemical identity only. Funding and conflicts: n/a Read: full-text
National Center for Biotechnology Information 2026 PubChem compound record for beta-pinene (CID 14896) PubChem REST API (retrieved 2026-10-25) · Source	Authoritative guidance n/a	n/a	n/a	CID 14896: 6,6-dimethyl-2-methylidenebicyclo[3.1.1]heptane, formula C10H16, molecular weight 136.23; CAS 127-91-3 in the synonym list. Identity confirmed Methods, limits and funding Outcomes: Chemical identity Limitations: Chemical identity only. Funding and conflicts: n/a Read: full-text
Porres-Martínez 2016 In vitro neuroprotective potential of the monoterpenes α-pinene and 1,8-cineole against H2O2-induced oxidative stress in PC12 cells. Z Naturforsch C J Biosci · PMID 27352445 · DOI	Laboratory research cell	Rat PC12 nerve-like cells under hydrogen peroxide stress	Alpha-pinene and 1,8-cineole Isolated compounds Pretreatment · vs Untreated cells	Pretreatment reduced the loss of cell viability, lowered free-radical production, raised antioxidant enzymes (catalase, SOD, GPx, GR, HO-1) and reduced a cell-death signal (caspase-3). Protection of cells in a dish against oxidative stress Methods, limits and funding Outcomes: Cell survival, free radicals, antioxidant enzymes, cell death Limitations: Cells in a dish; says nothing about effects in people or about doses reaching the brain. Funding and conflicts: Not stated in the abstract. Read: abstract
Rahimi 2023 The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats. Mol Biol Rep · PMID 37648947 · DOI	Animal research rat	Rats with ethanol-induced stomach damage	Alpha-pinene injected into the abdomen (i.p.) Isolated compound 10, 50 and 100 mg/kg Pretreatment · vs Control and omeprazole 20 mg/kg	Pretreatment reduced stomach damage, raised stomach pH like omeprazole, and raised Nrf2 and HO-1 activity. Damage was less than in controls at 50 and 100 mg/kg. Less alcohol-induced stomach damage in rats Methods, limits and funding Outcomes: Stomach lining damage, stomach pH, Nrf2 and HO-1 gene activity Limitations: Rats; injected rather than swallowed; not a test in people. Funding and conflicts: Not stated in the abstract. Read: abstract
Rivas da Silva 2012 Biological activities of α-pinene and β-pinene enantiomers. Molecules · PMID 22634841 · DOI	Laboratory research microbe and cell	Bacteria, fungi and mouse immune cells in the lab	Alpha-pinene and beta-pinene, positive (+) and negative (-) mirror forms Isolated compounds MIC 117 to 4,150 micrograms per mL for the (+) forms Time-kill curves up to 6 hours · vs Untreated and commercial antimicrobials	Only the (+) forms of alpha- and beta-pinene were active (MIC 117 to 4,150 micrograms per mL); the (-) forms were inactive up to 20 mg/mL. (+)-Alpha- and (+)-beta-pinene killed all Candida albicans within 60 minutes. With ciprofloxacin they were synergistic against MRSA. At 250 micrograms per mL they cut mouse macrophage viability to 66.8% and 57.7%. Lab antimicrobial activity of the (+) forms only; also toxic to immune cells at a modest concentration Methods, limits and funding Outcomes: Growth of microbes; synergy with drugs; toxicity to mouse macrophages Limitations: Test-tube work. Concentrations that kill microbes also reduced mammalian cell viability. Not a treatment test. Funding and conflicts: Not stated in the abstract. Read: abstract
Russo 2011 Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects. Br J Pharmacol · PMID 21749363 · DOI	Narrative review human and animal (review)	Review of cannabis terpenes and cannabinoids	Cannabis terpenoids including alpha-pinene (review)	The review names alpha-pinene among cannabis terpenoids, says terpenoids affect animal and even human behaviour when inhaled at blood levels in the single digits of ng per mL, and presents evidence for non-cannabinoid plant components as putative antidotes to the intoxicating effects of THC. A hypothesis paper, not a trial Methods, limits and funding Outcomes: Possible synergy between cannabis terpenes and cannabinoids Limitations: A 2011 review that proposed ideas to be tested. The abstract does not give pinene-specific results. Later testing of the memory idea for alpha-pinene is in the 2025 trial. Funding and conflicts: Not stated in the abstract. Read: abstract
Salehi 2019 Therapeutic Potential of α- and β-Pinene: A Miracle Gift of Nature. Biomolecules · PMID 31739596 · DOI	Review animal and human (review)	Review of studies on alpha- and beta-pinene	Alpha- and beta-pinene (review) Isolated compounds	The review lists reported activities including antibiotic resistance modulation, anticoagulant, antitumour, antimicrobial, antimalarial, antioxidant, anti-inflammatory, anti-Leishmania and analgesic effects, and summarises anxiolytic, gastroprotective, anticonvulsant and neuroprotective effects, with pancreatitis and pulpal pain, and discusses bioavailability and clinical applications. Broad list of reported activities, mostly from preclinical work Methods, limits and funding Outcomes: Pharmacological activities reported for the pinenes Limitations: A narrative review of mostly cell and animal research. The abstract gives no certainty rating or human trial results. Funding and conflicts: Not stated in the abstract. Read: abstract
Satou 2014 Daily inhalation of α-pinene in mice: effects on behavior and organ accumulation. Phytother Res · PMID 25340185 · DOI	Animal research mouse	Mice breathing alpha-pinene 90 minutes a day for 1, 3 or 5 days	Inhaled alpha-pinene Isolated compound 90 min per day for 1, 3 or 5 days · vs Water vapour	There was significant anxiolytic-like activity that stayed constant over the 5 days. Alpha-pinene in the brain and liver peaked on the third day. Anxiolytic-like behaviour in mice with daily inhalation Methods, limits and funding Outcomes: Elevated plus maze (anxiety-like behaviour); alpha-pinene in brain and liver Limitations: Mice only, one test of behaviour. Funding and conflicts: Not stated in the abstract. Read: abstract
Satyal 2017 Chemotypic Characterization and Biological Activity of Rosmarinus officinalis. Foods · PMID 28273883 · DOI	Lab analysis plant	Rosemary essential oils from Australia, USA, South Africa, Kenya, Nepal and Yemen	Rosemary (Rosmarinus officinalis) essential oils analysed by gas chromatography Essential oil	The oils were dominated by (+)-alpha-pinene (13.5% to 37.7%) and 1,8-cineole (16.1% to 29.3%). Antifungal, cytotoxicity and enzyme-inhibition screens showed only marginal activity. Rosemary oil is rich in (+)-alpha-pinene, with big differences between origins Methods, limits and funding Outcomes: Chemical make-up and screening activities Limitations: Six origins plus literature; five chemotypes were found, so one oil can differ a lot from another. Funding and conflicts: Not stated in the abstract. Read: abstract
Silva 2026 Assessment of serum concentrations of α-pinene, β-pinene, and limonene in the U.S. population from the 2013-2014 National Health and nutrition examination survey. J Chromatogr B Analyt Technol Biomed Life Sci · PMID 41365186 · DOI	Observational	2213 US population aged 6 to 80 (NHANES 2013-2014 surplus serum)	Blood levels of alpha-pinene, beta-pinene and limonene	Recent citrus-rich food intake was associated with 32.8% higher serum alpha-pinene (p = 0.0042). Hispanic and non-Hispanic Asian people had significantly higher serum beta-pinene than non-Hispanic White people. The abstract names cleaning products, fragrances, diet and drug use as exposure routes. Pinenes are measurable in the blood of ordinary people; diet is one source Methods, limits and funding Outcomes: Which factors go with higher blood terpene levels Limitations: Cross-sectional associations. The abstract does not say what blood levels mean for health. Funding and conflicts: Not stated in the abstract. Read: abstract
US Food and Drug Administration 2026 US Code of Federal Regulations Title 21, section 172.515: Synthetic flavoring substances and adjuvants eCFR (API; text version dated 2026-10-01, retrieved 2026-10-25) · Source	Authoritative guidance n/a	n/a	n/a	Section 172.515 says synthetic flavouring substances and adjuvants may be safely used in food when used in the minimum quantity required and according to good manufacturing practice, and lists "alpha-Pinene; 2-pinene" and "beta-Pinene; 2(10)-pinene" among them. Both pinenes are listed as permitted synthetic food flavourings Methods, limits and funding Outcomes: Whether the pinenes are on the FDA list of synthetic flavouring substances allowed in food Limitations: A food-additive listing with conditions. It does not address vaping, heating, concentrated oils or cannabis products. It is not the same as the "generally recognized as safe" list (section 182.60), where pinene does not appear. Funding and conflicts: n/a Read: full-text
Waidyanatha 2022 The common indoor air pollutant α-pinene is metabolised to a genotoxic metabolite α-pinene oxide. Xenobiotica · PMID 35473450 · DOI	Laboratory research human and rat cells and bacteria	Rat and human liver microsomes and hepatocytes; bacterial mutation tests	Alpha-pinene and alpha-pinene oxide Isolated compounds Up to 10,000 micrograms per plate	Alpha-pinene oxide was formed in rat and human microsomes and hepatocytes, with up to 4-fold higher formation in rats than humans. Alpha-pinene was not mutagenic in bacteria up to 10,000 micrograms per plate, whereas alpha-pinene oxide was mutagenic at 25 micrograms per plate and above. Human liver cells make alpha-pinene oxide; alpha-pinene itself was not mutagenic in bacteria Methods, limits and funding Outcomes: Formation of alpha-pinene oxide; mutagenicity Limitations: Test-tube work; the abstract does not describe real-world exposure risks in people. Funding and conflicts: US NIEHS / National Toxicology Program authors (listed in the record). Read: abstract
Waidyanatha 2026 Oral toxicokinetics of the indoor air pollutant, α-pinene, and its genotoxic metabolite, α-pinene oxide, in rodents and comparison to inhalation route of exposure. Toxicol Appl Pharmacol · PMID 42217586 · DOI	Animal research rat and mouse	Rats and mice given alpha-pinene by mouth for 7 days	Oral alpha-pinene and its metabolite alpha-pinene oxide Isolated compound Rats 10, 50 and 150 mg/kg; mice 50 mg/kg 7 days	Alpha-pinene was absorbed by mouth and turned into alpha-pinene oxide in both species. Oral bioavailability in rats was 22% to 49% in females and 5% to 33% in males depending on dose, and about 19% in mice. Both compounds reached mammary tissue. The authors say alpha-pinene by inhalation was associated with bladder hyperplasia and lower sperm counts in rodents. Alpha-pinene is absorbed by mouth in rodents and is partly converted to a reactive oxide Methods, limits and funding Outcomes: Blood levels (toxicokinetics) Limitations: Rodent study; the abstract does not tell what it means for people. Funding and conflicts: US NIEHS / National Toxicology Program authors (listed in the record). Read: abstract
Wang 2025 Effects of a cannabidiol/terpene formulation on sleep in individuals with insomnia: a double-blind, placebo-controlled, randomized, crossover study. J Clin Sleep Med · PMID 39167421 · DOI	Controlled trial	125 Adults with insomnia	Oral CBD plus eight terpenes: 1 mg each of linalool, myrcene, phytol, limonene, alpha-terpinene, alpha-terpineol, alpha-pinene and beta-caryophyllene Isolated compounds CBD 300 mg plus 1 mg of each terpene 4 weeks, at least 4 days per week, crossover · vs Placebo	The mix marginally increased slow-wave plus REM sleep versus placebo (mean 1.3%, standard error 0.60%, 95% CI 0.1 to 2.5%, P = .03). No effect on total sleep time or heart rate. No adverse events were reported. Small gain in one wrist-tracker measure for a nine-ingredient mixture Methods, limits and funding Outcomes: Percent of time in slow-wave plus REM sleep (wrist tracker) Limitations: A mixture: the effect cannot be credited to alpha-pinene. Wrist trackers are less accurate than sleep-lab tests. Funding and conflicts: Not stated in the abstract. Registration: ClinicalTrials.gov NCT05233761 Read: abstract
Yang 2016 α-Pinene, a Major Constituent of Pine Tree Oils, Enhances Non-Rapid Eye Movement Sleep in Mice through GABAA-benzodiazepine Receptors. Mol Pharmacol · PMID 27573669 · DOI	Animal research mouse	Mice (sleep recordings, brain-slice recordings, computer docking)	(-)-alpha-pinene by mouth Isolated compound · vs Control	(-)-alpha-pinene increased the duration of non-REM sleep and shortened sleep latency by oral dosing without changing REM sleep. It strengthened GABA-A receptor responses in brain slices, was mimicked by zolpidem and blocked by flumazenil, and the computer model placed it at the benzodiazepine binding site. More non-REM sleep in mice; acts at the GABA-A benzodiazepine site in the lab Methods, limits and funding Outcomes: Sleep stages by EEG and EMG, nerve-cell responses Limitations: Mouse and lab work only; the (-) form only. Not tested in people. Funding and conflicts: Not stated in the abstract. Read: abstract
de Barros 2025 α-Pinene and β-Pinene as Natural Adjuvants Against MRSA: Evidence from in vivo Models and Molecular Docking. Curr Microbiol · PMID 41231266 · DOI	Laboratory research microbe and insect larvae	Staphylococcus aureus in the lab and in Galleria mellonella wax-moth larvae	Alpha-pinene and beta-pinene alone and with antibiotics Isolated compounds MICs 64 to 4,096 micrograms per mL · vs Antibiotics alone	Beta-pinene showed pronounced synergy with amoxicillin and oxacillin; beta-pinene plus oxacillin cut bacterial load by 82.1% in the larvae model. No toxicity was seen in the larvae. Lab and insect-larvae synergy with antibiotics, strongest for beta-pinene Methods, limits and funding Outcomes: Bacterial growth, synergy, virulence assays, larval survival Limitations: Lab and larvae only; not a test in people. MIC values are high. Funding and conflicts: Not stated in the abstract. Read: abstract
de Groot 2016 Tea tree oil: contact allergy and chemical composition. Contact Dermatitis · PMID 27173437 · DOI	Review	Review of tea tree oil allergy and composition	Tea tree oil (alpha-pinene is one of its major constituents) Essential oil	Tea tree oil has caused more published allergic reactions than any other essential oil. Positive patch-test prevalence has ranged from 0.1% to 3.5%. Fresh oil is a weak to moderate sensitizer and oxidation increases its potency. The major sensitizers appear to be ascaridole, terpinolene, alpha-terpinene, 1,2,4-trihydroxymenthane, alpha-phellandrene and limonene. Co-reactivity to turpentine is frequent. Oxidised essential oils containing pinene are allergenic; pinene itself is not named as a main sensitizer here Methods, limits and funding Outcomes: Contact allergy to tea tree oil Limitations: Tea tree oil is a different product. It is included only because alpha-pinene is one of its constituents and because of the link to turpentine allergy. Funding and conflicts: Not stated in the abstract. Read: abstract

What we do not know yet

What the evidence supports: everything is against, very-low or insufficient certainty. Most is from animals, cells, whole oils or mixtures.
The only human test of the THC memory idea is a 19-person trial of vaporized alpha-pinene; it has not been repeated, and beta-pinene, oral products and real cannabis flower were not tested.
No trial tested alpha-pinene or beta-pinene alone for anxiety, sleep, mood, memory, airway disease or infection in people.
No human interaction study with prescription drugs and no data in pregnancy, breastfeeding, children or older adults.
Nothing is known about long-term inhalation of vaporized pinene, or whether pinene amounts on labels are accurate.
Our lab data do not say whether the (+) or (-) form was measured, and the datasets use different units.

How new research is added

New studies are found by a saved PubMed search and are placed in a review queue. A person reads each one before it changes any evidence rating or safety wording. New publications never change ratings automatically.

Lab markers

What changed in blood or saliva tests during trials. A changed number is not the same as feeling or being healthier, and we give no "normal ranges".

Other 15

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Working memory and thinking tests after THC

No change in trials

Computer tests of how well a person holds and uses information in mind, done before and after a dose.

Evidence against

Results and what it means

Why it was measured

THC is known to impair working memory, so this is the natural test of whether pinene reduces that impairment.

Statistical vs. clinical meaning

One small trial found no protective effect on the one outcome most claimed for pinene.

Safety implication

THC impairment is real and was not softened by pinene. Do not drive or do safety-critical tasks after THC.

Study	Result	People	Product
¹⁷	THC alone impaired working memory and cognitive performance versus placebo. Adding alpha-pinene (0.5, 5 or 15 mg) did not mitigate the impairment. Alpha-pinene alone (15 mg) did not differ from placebo.	19 healthy adults	Inhaled THC 30 mg with or without alpha-pinene, in a crossover design

¹⁷

●

How strong the high feels, and heart rate and blood pressure

No change in trials

Questionnaires on how a person feels (for example "high" or "drug effect") and measurements such as pulse and blood pressure.

Evidence against

Results and what it means

Why it was measured

It shows whether pinene changes the experience of THC or its effects on the body.

Statistical vs. clinical meaning

No sign that alpha-pinene makes THC feel milder or stronger in this small sample.

Safety implication

None reported for alpha-pinene at 15 mg alone.

Study	Result	People	Product
¹⁷	Adding alpha-pinene to THC did not significantly change the other subjective, cognitive or body effects measured. THC alone produced effects typical of THC-dominant cannabis.	19 healthy adults	Inhaled THC with or without alpha-pinene

¹⁷

↕

Behaviour in a raised maze (mice)

Mixed results in trials

A test in which mice choose between open and closed arms of a raised maze. More time in open arms is read as less anxious.

Very low certainty

Results and what it means

Why it was measured

It is a standard animal screen for anxiety-like behaviour.

Statistical vs. clinical meaning

Consistent with a calming-type effect at low levels in mice, but the effect changed with dose and the test is indirect.

Safety implication

None for people can be drawn.

Study	Result	People	Product
³⁶	Significant anxiolytic-like activity that stayed constant over 5 days of daily inhalation.	Mice	Inhaled alpha-pinene 90 minutes a day for 1, 3 or 5 days
¹⁵	A lower vapour level of cypress oil (rich in alpha-pinene) may give an anxiolytic-like effect and a high level an excitatory-like effect.	Mice	Inhaled hinoki cypress oil at 8 and 32 microlitres per litre
¹⁶	Sixty minutes of inhalation increased total distance travelled in the maze compared with control.	Mice	Inhaled alpha-pinene 10 microlitres per litre

^{36 15 16}

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Alpha-pinene in brain and liver tissue (mice)

Present in trials

How much alpha-pinene is found in organs after breathing it.

Very low certainty

Results and what it means

Why it was measured

It shows whether inhaled pinene reaches the brain and builds up with repeated use.

Statistical vs. clinical meaning

Pinene does enter the brain in mice. Amounts in people are not known from these studies.

Safety implication

Build-up in the liver was noted; the abstract does not report liver harm.

Study	Result	People	Product
³⁶	With daily 90-minute inhalation, alpha-pinene in the brain and liver peaked on the third day.	Mice	Inhaled alpha-pinene for 1, 3 or 5 days
¹⁵	Alpha-pinene was found in every brain region measured after breathing cypress oil vapour.	Mice	Inhaled cypress essential oil

^{36 15}

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Non-REM sleep time and time to fall asleep (mice)

Increased in trials

The length of the deeper non-REM stage of sleep, read from brain-wave recordings, and the delay before sleep starts.

Very low certainty

Results and what it means

Why it was measured

It is the direct test of a sedative-type effect on sleep.

Statistical vs. clinical meaning

A zolpidem-like pattern in mice, blocked by flumazenil, pointing to the benzodiazepine site.

Safety implication

Drowsiness could be expected if a similar effect existed in people; this is not shown.

Study	Result	People	Product
⁴³	(-)-alpha-pinene by mouth increased non-REM sleep time and reduced sleep latency without changing REM sleep.	Mice	(-)-alpha-pinene by mouth, with flumazenil and zolpidem comparisons

⁴³

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Share of the night in deep plus REM sleep (wrist tracker)

Increased in trials

The percentage of sleep time spent in slow-wave (deep) and REM sleep, estimated by a wrist-worn sleep tracker.

Very low certainty

Results and what it means

Why it was measured

It was the main outcome of the trial.

Statistical vs. clinical meaning

A marginal result for a mixture, not for pinene.

Safety implication

No adverse events were reported in the trial.

Study	Result	People	Product
⁴²	A small rise of 1.3% (95% CI 0.1 to 2.5%; P = .03) compared with placebo. No change in total sleep time, resting heart rate or heart rate variability.	125 adults with insomnia	Oral CBD 300 mg plus eight terpenes at 1 mg each, 4 weeks, crossover

⁴²

↕

Computer memory and attention test scores (rosemary scent)

Mixed results in trials

A set of nine computer tasks scored into six factors, including memory quality, secondary memory and speed.

Very low certainty

Results and what it means

Why it was measured

It gives objective scores of how a smell changes thinking.

Statistical vs. clinical meaning

Suggests rosemary scent can move some memory scores, but the active part was not shown to be pinene.

Safety implication

None reported.

Study	Result	People	Product
²⁵	Rosemary improved overall memory quality and secondary memory, and impaired speed of memory compared with controls.	144 healthy volunteers	Rosemary aroma versus lavender aroma versus none
²⁶	Task performance was related to the blood level of absorbed 1,8-cineole, with better performance at higher levels.	20 healthy volunteers	Rosemary aroma, one session

^{25 26}

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Immobility in the forced swimming test (mice)

Lowered in trials

How long a mouse stays still when placed in water. Less immobility is read as an antidepressant-like effect in screens.

Very low certainty

Results and what it means

Why it was measured

It is a common first screen for compounds that might affect mood.

Statistical vs. clinical meaning

An early screening signal for beta-pinene only.

Safety implication

None for people can be drawn.

Study	Result	People	Product
⁹	Beta-pinene and linalool reduced immobility; alpha-pinene did not.	Mice	Compounds injected into the abdomen
¹⁰	The reduced immobility with beta-pinene was blocked by drugs for 5-HT1A, beta-adrenergic and D1 receptors.	Mice	Beta-pinene with receptor-blocking drugs

^{9 10}

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Share of bronchitis patients who respond

Increased in trials

The share of patients whose signs and symptoms improve enough to continue without stopping treatment.

Very low certainty

Results and what it means

Why it was measured

It was the main outcome of the bronchitis trial.

Statistical vs. clinical meaning

A positive result for a three-terpene mixture, not for pinene alone.

Safety implication

The treatments were well tolerated, and there was no bronchoconstriction in any treatment group among continuing patients.

Study	Result	People	Product
²³	Responder rate at the first check was 92.9% with myrtol, 77.3% with placebo, 92.4% with cefuroxime and 89.6% with ambroxol. Placebo had more non-responders (20.9%) than myrtol (5.3%) at week 1.	676 adults with acute bronchitis	Standardized myrtol (alpha-pinene, limonene and 1,8-cineole) 300 mg four times daily for 14 days

²³

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Airway muscle contraction (isolated rat windpipe)

Increased in trials

How hard isolated windpipe rings contract when stimulated by acetylcholine or potassium.

Very low certainty

Results and what it means

Why it was measured

A standard way to test whether a molecule relaxes or tightens airway muscle.

Statistical vs. clinical meaning

The pinenes were not relaxant in this tissue test.

Safety implication

Cannot be applied to people from tissue work.

Study	Result	People	Product
²²	Alpha- and beta-pinene potentiated contractions caused by acetylcholine and by potassium. Whole eucalyptus oil relaxed the muscle.	Rat windpipe rings	Alpha- or beta-pinene, or Eucalyptus tereticornis oil, in a dish

²²

↕

Airway reactivity and inflammation markers (mice)

Mixed results in trials

How twitchy the airways are, plus lung inflammation markers such as MPO and cytokines.

Very low certainty

Results and what it means

Why it was measured

It shows whether inhaled oils calm or aggravate the lung.

Statistical vs. clinical meaning

Better airway function with more inflammation signals; the authors urge caution.

Safety implication

A reason for caution with concentrated pine oils in inflammation-related lung disease.

Study	Result	People	Product
³	Both Scots pine oil (39.4% alpha-pinene) and clove oil reduced airway hyperresponsiveness but increased MPO and several cytokines (IL-1 beta, KC, MCP-1, MIP-2, TNF-alpha).	Mice with endotoxin-induced lung inflammation	Inhaled Scots pine or clove oil

▼

Lowest concentration that stops microbes growing (MIC)

Lowered in trials

The smallest amount of a substance in a dish that stops a microbe from growing.

Very low certainty

Results and what it means

Why it was measured

It is the first screen for antimicrobial activity.

Statistical vs. clinical meaning

Weak to moderate lab activity at high concentrations; not a measure of treatment.

Safety implication

Active concentrations also reduced mouse macrophage viability (66.8% and 57.7% at 250 micrograms per mL).

Study	Result	People	Product
³³	MICs of 117 to 4,150 micrograms per mL for the (+) forms of alpha- and beta-pinene; no activity of the (-) forms up to 20 mg/mL.	Bacteria and fungi in culture	(+) and (-) alpha- and beta-pinene
⁴⁴	MICs of 64 to 4,096 micrograms per mL against Staphylococcus aureus; beta-pinene with oxacillin cut bacterial load by 82.1% in wax-moth larvae.	S. aureus and Galleria mellonella larvae	Alpha- or beta-pinene with antibiotics
¹³	MICs of 0.25 to 4.00 mg/mL for alpha- and beta-pinene mirror forms; combinations with other oil compounds differed by mirror form.	Microbes in culture	Mirror forms alone and with 14 oil compounds

^{33 44 13}

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Natural killer cell activity and urinary stress hormones

Increased in trials

How well a type of immune cell kills test cells in the lab, plus adrenaline and noradrenaline in urine as a stress signal.

Very low certainty

Results and what it means

Why it was measured

Earlier forest-trip studies reported changes in NK cells.

Statistical vs. clinical meaning

Interesting but not controlled; the clinical meaning is not known.

Safety implication

None reported.

Study	Result	People	Product
²¹	NK activity and NK, perforin, granulysin and granzyme-carrying cells rose; T cells and urinary adrenaline and noradrenaline fell compared with a normal day.	12 healthy men	Hinoki cypress oil vapour overnight for 3 nights

²¹

▼

Stomach lesions and stomach pH (rats)

Lowered in trials

The area of damage to the stomach lining and the acidity of stomach contents after an alcohol challenge.

Very low certainty

Results and what it means

Why it was measured

It measures protection from induced ulcers.

Statistical vs. clinical meaning

A rat finding only.

Safety implication

None for people can be drawn.

Study	Result	People	Product
³²	Less damage at 50 and 100 mg/kg of alpha-pinene (injected), and higher stomach pH, similar to omeprazole; Nrf2 and HO-1 genes were turned up.	Rats with ethanol-induced stomach damage	Alpha-pinene 10, 50 and 100 mg/kg into the abdomen

³²

▼

Cancer cell migration and colony formation

Lowered in trials

How far cancer cells move across a scratch and how many colonies they form in soft agar.

Not enough evidence

Results and what it means

Why it was measured

Standard lab screens for anti-cancer activity.

Statistical vs. clinical meaning

Lab screen only.

Safety implication

None for people can be drawn.

Study	Result	People	Product
⁷	Alpha-pinene with temozolomide reduced wound closure and colony formation more than either alone in LN229 and U87MG cells.	Human glioblastoma cell lines	Alpha-pinene with and without temozolomide

⁷

Safety

What studies and official sources say about side effects, medicines and who should check first. Each point shows what kind of evidence it comes from.

Main points to know 4

Turpentine and pine-type oils are known skin allergens, and alpha-pinene was a common culprit in patch tests. In Portugal, 17 of 22 people allergic to oil of turpentine reacted to alpha-pinene on patch testing, and 2 reacted to beta-pinene. In 24 pottery workers with hand dermatitis, 8 reacted to alpha-pinene. These are people already seen for turpentine allergy, so the rate in the general public is lower and unknown. A 2021 review says oxidised terpenes (hydroperoxides that form when terpenes sit in air) are a common cause of contact allergy, and that oxidation raises the allergy risk of essential oils such as tea tree oil, which contains alpha-pinene. The abstracts name delta-3-carene, limonene and linalool as the main oxidised allergens; which allergen caused each reaction to oxidised pinene was not shown in the abstracts we read. Observational ^{2 18 14 45 27 28}
Swallowing turpentine or pure pinene oils is dangerous, especially for children. Companies notified pure alpha-pinene as "may be fatal if swallowed and enters airways" in 74.1% of 2,036 reports and pure beta-pinene in 99.7% of 2,276 reports. A case report describes a 9-year-old boy who drank a turpentine hair-care preparation and needed two and a half days of hospital care for slow heart rate and low blood pressure; the report lists kidney failure, vision loss and death among possible signs of turpentine poisoning. These are company notifications and a single case, not regulator rulings, and turpentine is a mixture. Case report ^{27 28 11}
Breathing a lot of pinene vapour irritates the throat and airways. In chamber studies in healthy volunteers, five people exposed to alpha-pinene at up to 450 mg per cubic metre of air for 2 hours reported irritation, with a significant exposure-response relationship, although lung function 20 minutes later did not change. In eight men exposed to turpentine vapour (a mixture) at 450 mg per cubic metre, throat and airway discomfort and a rise in airway resistance were found. These levels are far above everyday air. A mouse study of Scots pine oil, which is 39.4% alpha-pinene, urges caution in inflammation-related lung disease. Pharmacokinetic study ^{5 6 3}
Heating and vaping: added terpenes raised breakdown products, and alpha-pinene forms a reactive oxide. In a laboratory test of cannabis vaporizing and dabbing, adding terpenes to THC raised gas-phase breakdown products compared with THC alone; the test used a terpene mixture, not pinene alone. In rat and human liver cells and microsomes, alpha-pinene is turned into alpha-pinene oxide, which was mutagenic in bacteria at much lower concentrations than alpha-pinene itself. Rodent inhalation studies reported bladder hyperplasia and lower sperm counts. What these findings mean for people who vaporize cannabis is not known. Lab analysis ^{24 40 41}

Side effects 5

Effect	How often	Severity and recovery	Evidence
Allergic contact dermatitis (itchy, red rash) from turpentine or pinene-containing products These are patients selected because they were allergic to turpentine. Fresh and oxidised material may differ.	17 of 22 turpentine-allergic patients in Portugal reacted to alpha-pinene; 8 of 24 pottery workers; no rate for the general public	moderate In pottery workers, the problem eased after returning to a different turpentine; otherwise not described	Observational ^{2 18}
Throat and airway irritation when breathing high vapour levels Chamber exposures were much higher than the levels people meet at home or from a vaporized cannabis puff.	Five of the chamber volunteers reported irritation at alpha-pinene up to 450 mg per cubic metre; number of volunteers not given in the abstract	mild Lung function was unchanged 20 minutes after a 2-hour exposure to alpha-pinene; airway resistance rose after turpentine exposure in 8 men	Pharmacokinetic study ^{5 6}
Poisoning if turpentine or concentrated pine oil is swallowed (coughing, aspiration, low blood pressure, slow heart rate) Turpentine is a mixture; the report does not isolate pinene.	One child case report; no rate	severe The child recovered after hospital care; the report also lists deaths among possible outcomes	Case report ^{11 27}
Possible effects on the bladder and on sperm counts (rodents breathing alpha-pinene) Reported in rodents after inhalation. No human finding of this kind was found in the abstracts we read.	Not applicable (animal finding)	not rated Not described in the abstracts	Animal research ^{41 40}
Adverse events from alpha-pinene alone: none described in the abstract of the one THC trial The trial gave alpha-pinene 15 mg once by vaporizer. It tells us nothing about repeated or long-term use.	The abstract of the 19-person trial does not list adverse events	not rated Not applicable	Controlled trial ¹⁷

Liver

No human case of liver injury from pinene was found in the sources read, and LiverTox was not read. In mice, alpha-pinene breathed daily built up in the liver as well as the brain, peaking on the third day. Human liver cells and liver microsomes turn alpha-pinene into alpha-pinene oxide; formation was up to 4-fold higher in rat than in human preparations. The abstracts do not report liver harm. ^{36 40}

How often. No human rate was found.

Monitoring. No agency monitoring advice was read.

This is a safety connection. It is not evidence that Pinene (Alpha-Pinene and Beta-Pinene) supports liver health.

Medicines and supplements 4

With	Why it matters	Importance	Evidence	What to do
THC (cannabis) cannabis	In a 19-person trial, inhaled alpha-pinene (0.5, 5 or 15 mg) did not reduce THC memory impairment and did not significantly change THC's other subjective, cognitive or body effects.	Not shown to make THC safer or milder. Beta-pinene was not tested.	Controlled trial Low certainty ¹⁷	Do not assume pinene protects against THC. Impairment from THC still applies; do not drive after THC.
Sedatives, sleep medicines, alcohol and other drugs that cause drowsiness sedatives	In mice, (-)-alpha-pinene by mouth increased non-REM sleep through the benzodiazepine site of the GABA-A receptor, and the effect was blocked by flumazenil and mimicked by zolpidem.	Not studied in people. If a similar effect existed, additive drowsiness would be a concern.	Animal research Very low certainty ⁴³	Ask a doctor or pharmacist before combining concentrated pinene products with sedatives.
Antibiotics drug	In the lab, beta-pinene strengthened amoxicillin and oxacillin against Staphylococcus aureus, and (+)-pinenes were synergistic with ciprofloxacin against MRSA.	Laboratory finding only. No human data. It does not mean pinene should be added to antibiotics.	Laboratory research Very low certainty ^{33 44}	Do not use pinene products in place of, or alongside, prescribed antibiotics unless your clinician agrees.
Medicines broken down by liver enzymes (CYP drugs) drug	No human interaction study or enzyme-inhibition study of the pinenes was found. Human liver cells turn alpha-pinene into alpha-pinene oxide.	Unknown.	Laboratory research Not enough evidence ⁴⁰	Unknown. A doctor or pharmacist can check your list of medicines.

Who should check first 6

Situation	Advice	Evidence
Known turpentine, fragrance or pine-oil allergy, or hand dermatitis	Avoid skin contact with turpentine and pine or pinene-rich oils, especially old oils that have sat in air. Ask a dermatologist about patch testing.	Observational ^{2 18 14}
Asthma, chronic lung disease or a sensitive airway	Concentrated vapour irritated the airways of volunteers, and a mouse study of pine oil showed more inflammation signals. Ask a doctor before breathing concentrated oils or vaporized terpenes.	Pharmacokinetic study ^{5 6 3}
Pregnancy and breastfeeding	No human data on pinene were found. In rodents, alpha-pinene and alpha-pinene oxide were found in mammary tissue after oral dosing. THC carries its own risks in pregnancy and breastfeeding (see the THC page). Ask a doctor or midwife.	Animal research ⁴¹
Children	Swallowing turpentine, pine oil cleaners or pure terpene oils can be dangerous for children. Keep them out of reach. No data on pinene taken on purpose by children were found.	Case report ^{11 27}
Teenagers, young adults and older adults using cannabis	No data on pinene alone in these groups. The risks of THC (impairment, anxiety, psychosis risk in vulnerable people, dependence) apply to cannabis products whatever their pinene content; see the THC page.	Not studied ¹⁷
Before driving or operating machinery after cannabis	THC impaired thinking and working memory in the trial and alpha-pinene did not prevent it. Do not drive after THC.	Controlled trial ¹⁷

Overdose, product quality and regulators

Summary. Pinene is a common natural molecule and a permitted food flavour in the US. The best-documented harms in people are skin allergy to turpentine-type material, airway irritation at high vapour levels and poisoning if turpentine is swallowed. Animal studies raise questions about inhaled alpha-pinene, but what they mean for people is not established.

Animal. Rodents breathing alpha-pinene showed bladder hyperplasia and lower sperm counts, according to the 2022 and 2026 toxicology papers. Alpha-pinene was absorbed by mouth (rat bioavailability 22 to 49% in females and 5 to 33% in males depending on dose; about 19% in mice) and was turned into alpha-pinene oxide, which was distributed to mammary tissue. Brine shrimp: S-(-)-beta-pinene caused 90.82% deaths versus 30.75% for R-(+)-beta-pinene, and both alpha-pinene forms were non-toxic in that rough screen.

Human doses studied. About 10, 225 and 450 mg per cubic metre of air for 2 hours in chamber studies; 15 mg alpha-pinene by vaporizer once; 1 mg alpha-pinene a day inside a nine-ingredient mixture for 4 weeks.

High dose or long term signals. Throat and airway irritation at 450 mg per cubic metre. No long-term human data on pure pinene.

Overdose response. A child who drank a turpentine preparation had slow heart rate and low blood pressure and needed hospital care for two and a half days. Company notifications list aspiration hazard for both pinenes.

Genotoxicity. Alpha-pinene was not mutagenic in bacteria up to 10,000 micrograms per plate, but its metabolite alpha-pinene oxide was mutagenic at 25 micrograms per plate and above. The amount of oxide formed from alpha-pinene in the bacterial test was about 3-fold below the lowest level of oxide that was positive, which may explain why alpha-pinene itself was negative.

Pinene is added to foods and fragrances as well as occurring naturally, so a pinene figure on a label does not show whether it came from the plant. We found no study that checked whether the pinene figure on cannabis product labels is accurate. Pinene amounts in the same strain vary a lot between plants and batches.

Natural variation. Ten plants of one strain grown together varied by about 20% in terpenes above 20 micrograms per gram, and a 2025 analysis of 140 medicinal flowers found terpene profiles did not match sativa, indica or hybrid labels. Our lab numbers on the Overview tab show the same strain name measured very differently in different datasets. ^{8 12}

Heated products. In a lab test of vaporizers and dabbing, adding terpenes to THC raised gas-phase breakdown products compared with THC alone. The test used a terpene mixture, not pinene alone. ²⁴

Exposure from everyday sources. In blood samples from 2,213 people in the US national survey (2013-2014), serum alpha-pinene was 32.8% higher with recent citrus-rich food intake, and beta-pinene differed by ethnic group. The authors list cleaning products, fragrances, diet and drug use as exposure routes. The abstract does not say what the levels mean for health. ³⁸

Body	Action	Date
US Food and Drug Administration (Code of Federal Regulations, Title 21, section 172.515) (United States)	Lists "alpha-Pinene; 2-pinene" and "beta-Pinene; 2(10)-pinene" among synthetic flavouring substances and adjuvants that may be safely used in food when used in the minimum quantity required and in line with good manufacturing practice. source	2026-10-25
Company notifications to the European Chemicals Agency classification inventory (as aggregated by PubChem) (European Union (notifications))	Alpha-pinene: 97.6% of 2,036 reports notify flammable liquid and vapour, 83.2% skin irritation, 66.1% skin sensitisation and 74.1% aspiration hazard. Beta-pinene: 99.7% of 2,276 reports notify flammable liquid and aspiration hazard and 97.5% skin sensitisation. source	2026-10-25

When to get medical help

A child or adult swallowed turpentine, pine oil cleaner or a pure terpene oil and is coughing, choking, wheezing, vomiting or has trouble breathing: get emergency help and call a poison centre (in the US, 1-800-222-1222).
A rash that spreads quickly, blisters, or swells the face or lips after skin contact with turpentine or an oil containing pinene.
Chest tightness, wheeze or breathlessness after breathing concentrated oil vapour or a vaporized product.
Very drowsy, confused, with a slow heartbeat or fainting after swallowing any terpene oil.

Stop using the product and contact a clinician or emergency service. In the US, Poison Control is 1-800-222-1222.

General information only, not medical advice. Ask a clinician or pharmacist before use, especially with medicines, in pregnancy or with a health condition. See the Natural Remedy Safety guide.

Frequently asked questions

What is pinene?

Pinene is the name for two pine-smelling plant molecules, alpha-pinene and beta-pinene. Both are monoterpenes with the formula C10H16. They are found in pine and rosemary oils, in other plants and in cannabis.

^{29 30 37}

Are alpha-pinene and beta-pinene the same?

No. They have the same formula but different structures and have been studied separately. Most human work is on alpha-pinene. In mice only beta-pinene was antidepressant-like in one test, and each has two mirror forms that can behave differently.

^{29 30 9 13}

Which plants contain pinene?

Pine and rosemary oils are rich in alpha-pinene: one Scots pine oil was 39.4% and rosemary oils from six places were 13.5% to 37.7%. It is also in hinoki cypress oil, tea tree oil, turpentine and cannabis. Amounts vary by plant and batch.

^{3 37 15 45 4}

Does pinene counter the memory effects of THC?

Not in the one human trial. In 19 healthy adults, inhaled alpha-pinene did not reduce THC memory impairment or change other THC effects. Beta-pinene was not tested. Do not drive after THC.

¹⁷

Does pinene help anxiety, sleep or memory?

Evidence in people is lacking. Mice breathing alpha-pinene showed anxiolytic-like behaviour, and (-)-alpha-pinene by mouth lengthened their non-REM sleep. Rosemary scent changed some memory scores in people, but whole oil was used. It is not a treatment for any condition.

^{36 43 25 26}

Does pinene open the airways or treat bronchitis?

Not shown for pinene alone. A distillate of alpha-pinene, limonene and 1,8-cineole beat placebo in acute bronchitis, but the effect cannot be credited to pinene. In isolated rat windpipe, pinenes made muscle contract more, and high vapour levels irritated volunteers' airways.

^{23 22 5 6}

Is pinene an antibiotic?

Only in the lab. The (+) forms of alpha- and beta-pinene killed bacteria and fungi in test tubes at high concentrations that also reduced mouse immune-cell survival, and beta-pinene boosted some antibiotics against Staphylococcus aureus. Do not use it on infections.

^{33 44}

Can pinene cause a skin allergy?

Yes. In patch tests of turpentine-allergic people, 17 of 22 reacted to alpha-pinene in Portugal and 8 of 24 pottery workers did. Oxidised terpenes are a recognised cause of contact allergy. If a product makes your skin itch or blister, stop using it and ask a clinician.

^{2 18 14}

Is pinene safe?

It is a permitted food flavour in the US, but concentrated pinene is a hazard: it is flammable, irritates the skin and can be dangerous if swallowed. High vapour levels irritated the airways of volunteers. Long-term safety of vaporized pinene is unknown, and added terpenes raised breakdown products in a lab test of vape and dab aerosol.

^{39 27 28 5 24}

Do "pine" strains have more pinene?

We have no evidence that a strain name or smell describes its pinene content. Our lab data show the same strain measured very differently between datasets, and 140 German flowers showed no link between terpene profiles and sativa, indica or hybrid labels.

^{12 8}

Is pinene safe in pregnancy, for children or for older adults?

We found no human data for these groups. Children can be seriously harmed by swallowing turpentine or pine-oil products. In rodents, alpha-pinene reached mammary tissue. Ask your doctor or pharmacist before using essential oils, supplements or cannabis products.

^{11 41}

Is pinene approved or regulated?

In the US, alpha-pinene and beta-pinene are listed among synthetic flavouring substances that may be safely used in food under stated conditions. That is a food flavour status and says nothing about vaping, heating or cannabis products. Companies also notify pinene as a skin irritant and sensitiser.

^{39 27 28}

Explore by health goal

Editorial transparency

Written by: AlternativeMed editorial team. Research compiled with AI assistance, then checked against PubMed records.
Medical review: None. This page has not been reviewed by a physician or pharmacist.
First published: 2026-10-25
Last substantive update: 2026-10-25
How we research: We searched PubMed and read abstracts of about 40 sources, read the FDA flavouring list through the Electronic Code of Federal Regulations, read PubChem for identity and hazard classifications, and ran the PubChem identity check. Only abstracts were read for papers. Certainty ratings are the compiler's own reading. EFSA, JECFA, IFRA, EU cosmetics rules, LiverTox and LactMed were not read. The lab numbers are from public regulator datasets and published papers, not our own testing.
How we rate evidence: See "How we rate evidence" in the Evidence tab.
Independence: No product is promoted on this page. No affiliate links are shown, and none will be added for pinene. This page is information, not a recommendation to use it.

Sources 45

Each source was opened and checked. Where we read only the abstract, it says so. The small numbers in the text point here.

Booth JK, Page JE, Bohlmann J. Terpene synthases from Cannabis sativa.. PLoS One. 2017. PMID 28355238. DOI 10.1371/journal.pone.0173911. (abstract)
Cachão P, Menezes Brandão F, Carmo M, et al. Allergy to oil of turpentine in Portugal.. Contact Dermatitis. 1986. PMID 3720296. DOI 10.1111/j.1600-0536.1986.tb01225.x. (abstract)
Csikós E, Csekő K, Kemény Á, et al. Pinus sylvestris L. and Syzygium aromaticum (L.) Merr. & L. M. Perry Essential Oils Inhibit Endotoxin-Induced Airway Hyperreactivity despite Aggravated Inflammatory Mechanisms in Mice.. Molecules. 2022. PMID 35744988. DOI 10.3390/molecules27123868. (abstract)
Dei Cas M, Arnoldi S, Monguzzi L, et al. Characterization of chemotype-dependent terpenoids profile in cannabis by headspace gas-chromatography coupled to time-of-flight mass spectrometry.. J Pharm Biomed Anal. 2021. PMID 34111731. DOI 10.1016/j.jpba.2021.114180. (abstract)
Falk AA, Hagberg MT, Löf AE, et al. Uptake, distribution and elimination of alpha-pinene in man after exposure by inhalation.. Scand J Work Environ Health. 1990. PMID 2255878. DOI 10.5271/sjweh.1771. (abstract)
Filipsson AF. Short term inhalation exposure to turpentine: toxicokinetics and acute effects in men.. Occup Environ Med. 1996. PMID 8777445. DOI 10.1136/oem.53.2.100. (abstract)
Gautam M, Gabrani R. Comparative analysis of α-pinene alone and combined with temozolomide in human glioblastoma cells.. Nat Prod Res. 2024. PMID 37665021. DOI 10.1080/14786419.2023.2252152. (abstract)
Giovannoni S, Lancioni C, Vaccarini C, et al. Determination of variability of terpenes and terpenoids in Cannabis sativa by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry.. J Chromatogr A. 2023. PMID 36463647. DOI 10.1016/j.chroma.2022.463669. (abstract)
Guzmán-Gutiérrez SL, Gómez-Cansino R, García-Zebadúa JC, et al. Antidepressant activity of Litsea glaucescens essential oil: identification of β-pinene and linalool as active principles.. J Ethnopharmacol. 2012. PMID 22867633. DOI 10.1016/j.jep.2012.07.026. (abstract)
Guzmán-Gutiérrez SL, Bonilla-Jaime H, Gómez-Cansino R, et al. Linalool and β-pinene exert their antidepressant-like activity through the monoaminergic pathway.. Life Sci. 2015. PMID 25771248. DOI 10.1016/j.lfs.2015.02.021. (abstract)
Güzel A, Açıkgöz M. A lethal danger in the home: turpentine poisoning.. Turk J Pediatr. 2015. PMID 26690600. (abstract)
Herwig N, Utgenannt S, Nickl F, et al. Classification of Cannabis Strains Based on their Chemical Fingerprint-A Broad Analysis of Chemovars in the German Market.. Cannabis Cannabinoid Res. 2025. PMID 39137353. DOI 10.1089/can.2024.0127. (abstract)
Hoosen N, Viljoen A, van Vuuren S. Stereoisomeric interactions of α- and β-pinene with essential oil compounds.. Fitoterapia. 2026. PMID 41253204. DOI 10.1016/j.fitote.2025.106989. (abstract)
Karlberg AT, Lepoittevin JP. One hundred years of allergic contact dermatitis due to oxidized terpenes: What we can learn from old research on turpentine allergy.. Contact Dermatitis. 2021. PMID 34453446. DOI 10.1111/cod.13962. (abstract)
Kasuya H, Iida S, Ono K, et al. Intracerebral Distribution of a-Pinene and the Anxiolytic-like Effect in Mice Following Inhaled Administration of Essential Oil from Chamaecyparis obtuse.. Nat Prod Commun. 2015. PMID 26434146. (abstract)
Kasuya H, Okada N, Kubohara M, et al. Expression of BDNF and TH mRNA in the brain following inhaled administration of α-pinene.. Phytother Res. 2015. PMID 25230317. DOI 10.1002/ptr.5224. (abstract)
Kumar L, Spindle TR, Zamarripa CA, et al. The Individual and Interactive Effects of Alpha-Pinene and Delta-9-Tetrahydrocannabinol in Healthy Adults.. Med Cannabis Cannabinoids. 2025. PMID 40734690. DOI 10.1159/000547014. (abstract)
Lear JT, Heagerty AH, Tan BB, et al. Transient re-emergence of oil of turpentine allergy in the pottery industry.. Contact Dermatitis. 1996. PMID 8930478. DOI 10.1111/j.1600-0536.1996.tb02336.x. (abstract)
Levin JO, Eriksson K, Falk A, et al. Renal elimination of verbenols in man following experimental alpha-pinene inhalation exposure.. Int Arch Occup Environ Health. 1992. PMID 1587632. DOI 10.1007/BF00386348. (abstract)
Lewis MA, Russo EB, Smith KM. Pharmacological Foundations of Cannabis Chemovars.. Planta Med. 2018. PMID 29161743. DOI 10.1055/s-0043-122240. (abstract)
Li Q, Kobayashi M, Wakayama Y, et al. Effect of phytoncide from trees on human natural killer cell function.. Int J Immunopathol Pharmacol. 2009. PMID 20074458. DOI 10.1177/039463200902200410. (abstract)
Lima FJ, Brito TS, Freire WB, et al. The essential oil of Eucalyptus tereticornis, and its constituents alpha- and beta-pinene, potentiate acetylcholine-induced contractions in isolated rat trachea.. Fitoterapia. 2010. PMID 20302920. DOI 10.1016/j.fitote.2010.03.012. (abstract)
Matthys H, de Mey C, Carls C, et al. Efficacy and tolerability of myrtol standardized in acute bronchitis. A multi-centre, randomised, double-blind, placebo-controlled parallel group clinical trial vs. cefuroxime and ambroxol.. Arzneimittelforschung. 2000. PMID 10994153. DOI 10.1055/s-0031-1300276. (abstract)
Meehan-Atrash J, Luo W, McWhirter KJ, et al. Aerosol Gas-Phase Components from Cannabis E-Cigarettes and Dabbing: Mechanistic Insight and Quantitative Risk Analysis.. ACS Omega. 2019. PMID 31592479. DOI 10.1021/acsomega.9b02301. (abstract)
Moss M, Cook J, Wesnes K, et al. Aromas of rosemary and lavender essential oils differentially affect cognition and mood in healthy adults.. Int J Neurosci. 2003. PMID 12690999. DOI 10.1080/00207450390161903. (abstract)
Moss M, Oliver L. Plasma 1,8-cineole correlates with cognitive performance following exposure to rosemary essential oil aroma.. Ther Adv Psychopharmacol. 2012. PMID 23983963. DOI 10.1177/2045125312436573. (abstract)
National Center for Biotechnology Information, PubChem (company notifications to the ECHA C&L Inventory). PubChem GHS classification summary for alpha-pinene (CID 6654). PubChem PUG-View API (retrieved 2026-10-25). 2026. (full-text)
National Center for Biotechnology Information, PubChem (company notifications to the ECHA C&L Inventory). PubChem GHS classification summary for beta-pinene (CID 14896). PubChem PUG-View API (retrieved 2026-10-25). 2026. (full-text)
National Center for Biotechnology Information, PubChem. PubChem compound record for alpha-pinene (CID 6654), with the (+) form CID 82227 and the (-) form CID 440968. PubChem REST API (retrieved 2026-10-25). 2026. (full-text)
National Center for Biotechnology Information, PubChem. PubChem compound record for beta-pinene (CID 14896). PubChem REST API (retrieved 2026-10-25). 2026. (full-text)
Porres-Martínez M, González-Burgos E, Carretero ME, et al. In vitro neuroprotective potential of the monoterpenes α-pinene and 1,8-cineole against H2O2-induced oxidative stress in PC12 cells.. Z Naturforsch C J Biosci. 2016. PMID 27352445. DOI 10.1515/znc-2014-4135. (abstract)
Rahimi K, Shirvani N, Sanaie P, et al. The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats.. Mol Biol Rep. 2023. PMID 37648947. DOI 10.1007/s11033-023-08765-y. (abstract)
Rivas da Silva AC, Lopes PM, Barros de Azevedo MM, et al. Biological activities of α-pinene and β-pinene enantiomers.. Molecules. 2012. PMID 22634841. DOI 10.3390/molecules17066305. (abstract)
Russo EB. Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects.. Br J Pharmacol. 2011. PMID 21749363. DOI 10.1111/j.1476-5381.2011.01238.x. (abstract)
Salehi B, Upadhyay S, Erdogan Orhan I, et al. Therapeutic Potential of α- and β-Pinene: A Miracle Gift of Nature.. Biomolecules. 2019. PMID 31739596. DOI 10.3390/biom9110738. (abstract)
Satou T, Kasuya H, Maeda K, et al. Daily inhalation of α-pinene in mice: effects on behavior and organ accumulation.. Phytother Res. 2014. PMID 25340185. DOI 10.1002/ptr.5105. (abstract)
Satyal P, Jones TH, Lopez EM, et al. Chemotypic Characterization and Biological Activity of Rosmarinus officinalis.. Foods. 2017. PMID 28273883. DOI 10.3390/foods6030020. (abstract)
Silva L, Newman C, Bhandari D, et al. Assessment of serum concentrations of α-pinene, β-pinene, and limonene in the U.S. population from the 2013-2014 National Health and nutrition examination survey.. J Chromatogr B Analyt Technol Biomed Life Sci. 2026. PMID 41365186. DOI 10.1016/j.jchromb.2025.124873. (abstract)
US Food and Drug Administration, via the Electronic Code of Federal Regulations. US Code of Federal Regulations Title 21, section 172.515: Synthetic flavoring substances and adjuvants. eCFR (API; text version dated 2026-10-01, retrieved 2026-10-25). 2026. (full-text)
Waidyanatha S, Black SR, Witt KL, et al. The common indoor air pollutant α-pinene is metabolised to a genotoxic metabolite α-pinene oxide.. Xenobiotica. 2022. PMID 35473450. DOI 10.1080/00498254.2022.2070047. (abstract)
Waidyanatha S, Fennell TR, Black SR, et al. Oral toxicokinetics of the indoor air pollutant, α-pinene, and its genotoxic metabolite, α-pinene oxide, in rodents and comparison to inhalation route of exposure.. Toxicol Appl Pharmacol. 2026. PMID 42217586. DOI 10.1016/j.taap.2026.117893. (abstract)
Wang M, Faust M, Abbott S, et al. Effects of a cannabidiol/terpene formulation on sleep in individuals with insomnia: a double-blind, placebo-controlled, randomized, crossover study.. J Clin Sleep Med. 2025. PMID 39167421. DOI 10.5664/jcsm.11324. (abstract)
Yang H, Woo J, Pae AN, et al. α-Pinene, a Major Constituent of Pine Tree Oils, Enhances Non-Rapid Eye Movement Sleep in Mice through GABAA-benzodiazepine Receptors.. Mol Pharmacol. 2016. PMID 27573669. DOI 10.1124/mol.116.105080. (abstract)
de Barros AV, de Veras BO, de Lima Menezes G, et al. α-Pinene and β-Pinene as Natural Adjuvants Against MRSA: Evidence from in vivo Models and Molecular Docking.. Curr Microbiol. 2025. PMID 41231266. DOI 10.1007/s00284-025-04593-4. (abstract)
de Groot AC, Schmidt E. Tea tree oil: contact allergy and chemical composition.. Contact Dermatitis. 2016. PMID 27173437. DOI 10.1111/cod.12591. (abstract)

Education, not medical advice. This page is for general information. It does not diagnose or treat any condition and is not a substitute for care from a qualified health professional. Natural does not automatically mean safe. Ask a clinician or pharmacist before changing your care, especially if you take medication, are pregnant or breastfeeding, or have a health condition.